Transcriptional inhibition of feline immunodeficiency virus by alpha-amanitin.

ABSTRACT: Alpha-amanitin, one of the amatoxins in egg amanita, has a cyclic peptide structure, and was reported as having antiviral activity against several viruses. We investigated whether ?-amanitin has antiviral activity against feline immunodeficiency virus (FIV). FL-4 cells persistently infected with FIV Petaluma were cultured with ?-amanitin. Reverse transcriptase (RT) activity in the supernatant of FL-4 cells was significantly inhibited by ?-amanitin. In addition, the production of FIV core protein in FL-4 cells was inhibited by ?-amanitin when analyzed by western blotting. Furthermore, ?-amanitin inhibited the transcription of FIV in real-time RT-PCR. These data suggested that ?-amanitin showed anti-FIV activity by inhibiting the RNA transcription level.

SUBMITTER: Tanabe T 

PROVIDER: S-EPMC7870409 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature