Ontology highlight
ABSTRACT:
SUBMITTER: Poddar A
PROVIDER: S-EPMC7870926 | biostudies-literature | 2021 Feb
REPOSITORIES: biostudies-literature
Nature communications 20210208 1
Base-pairing interactions mediate many intermolecular target recognition events. Even a single base-pair mismatch can cause a substantial difference in activity but how such changes influence the target search kinetics in vivo is unknown. Here, we use high-throughput sequencing and quantitative super-resolution imaging to probe the mutants of bacterial small RNA, SgrS, and their regulation of ptsG mRNA target. Mutations that disrupt binding of a chaperone protein, Hfq, and are distal to the mRNA ...[more]