CoronaHiT: high-throughput sequencing of SARS-CoV-2 genomes.
Ontology highlight
ABSTRACT:
SUBMITTER: Baker DJ
PROVIDER: S-EPMC7871948 | biostudies-literature | 2021 Feb
REPOSITORIES: biostudies-literature
Publications
CoronaHiT: high-throughput sequencing of SARS-CoV-2 genomes.
Baker Dave J DJ Aydin Alp A Le-Viet Thanh T Kay Gemma L GL Rudder Steven S de Oliveira Martins Leonardo L Tedim Ana P AP Kolyva Anastasia A Diaz Maria M Alikhan Nabil-Fareed NF Meadows Lizzie L Bell Andrew A Gutierrez Ana Victoria AV Trotter Alexander J AJ Thomson Nicholas M NM Gilroy Rachel R Griffith Luke L Adriaenssens Evelien M EM Stanley Rachael R Charles Ian G IG Elumogo Ngozi N Wain John J Prakash Reenesh R Meader Emma E Mather Alison E AE Webber Mark A MA Dervisevic Samir S Page Andrew J AJ O'Grady Justin J
Genome medicine 20210209 1
We present CoronaHiT, a platform and throughput flexible method for sequencing SARS-CoV-2 genomes (≤ 96 on MinION or > 96 on Illumina NextSeq) depending on changing requirements experienced during the pandemic. CoronaHiT uses transposase-based library preparation of ARTIC PCR products. Method performance was demonstrated by sequencing 2 plates containing 95 and 59 SARS-CoV-2 genomes on nanopore and Illumina platforms and comparing to the ARTIC LoCost nanopore method. Of the 154 samples sequenced ...[more]
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