Dataset Information

Comparative genomic analysis of superoxide dismutase (SOD) genes in three Rosaceae species and expression analysis in Pyrus bretschneideri.

ABSTRACT: Superoxide dismutases (SODs) are antioxidant enzymes that play a critical role in the polymerization of lignin monomers. Although current research has indicated that SODs are involved in plant growth and development, information on SODs in pear (Pyrus bretschneideri) and their function in lignin formation is scarce. In this study, 25 SODs, containing three kinds of plant SODs (Cu/Zn-SODs, Mn-SODs, and Fe-SODs), were identified from three Rosaceae species, and 11 of these genes were found in pear. According to the evolutionary analysis, the genes were divided into four subgroups, the division of which is consistent with the intron-exon and conserved motif analyses. These PbSODs were randomly scattered across 7 chromosomes. We have analysed the conserved domains and gene family evolution and predicted the cis-elements of the promoter. Ka/Ks analysis pointed that SOD genes mainly underwent purifying selection. Subsequently, the expression patterns of 11 PbSODs were examined in different tissues, at different developmental periods, in different pear varieties and under different hormone treatments. Gene expression analysis showed that PbCSD3 exhibited transcript levels consistent with the typical changes in lignin content. The changes in SOD activity and hydrogen peroxide (H₂O₂) content combined with the results of a spatio-temporal expression analysis showed that PbCSD3 was a candidate gene in reactive oxygen species (ROS) metabolism during the lignification of pear stone cells. Thus, our research reveals the evolutionary features of the SOD family in Rosaceae species and provide useful information for analysis of functional genome of the SOD family in pear.

Supplementary information

The online version of this article (10.1007/s12298-021-00926-2) contains supplementary material, which is available to authorized users.

SUBMITTER: Li G

PROVIDER: S-EPMC7873169 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:The INDETERMINATE DOMAIN (IDD) gene family encodes hybrid transcription factors with distinct zinc finger motifs and appears to be found in all higher plant genomes. IDD genes have been identified throughout the genomes of the model plants Arabidopsis thaliana and Oryza sativa, and the functions of many members of this gene family have been studied. However, few studies have investigated the IDD gene family in Rosaceae species (among these species, a genome-wide identification of the IDD gene family has only been completed in Malus domestica). This study focuses on a comparative genomic analysis of the IDD gene family in five Rosaceae species (Pyrus bretschneideri, Fragaria vesca, Prunus mume, Rubus occidentalis and Prunus avium). We identified a total of 68 IDD genes: 16 genes in Chinese white pear, 14 genes in F. vesca, 13 genes in Prunus mume, 14 genes in R. occidentalis and 11 genes in Prunus avium. The evolution of the IDD genes in these five Rosaceae species was revealed by constructing a phylogenetic tree, tracking gene duplication events, and performing a sliding window analysis and a conserved microsynteny analysis. The expression analysis of different organs showed that most of the pear IDD genes are found at a very high transcription level in fruits, flowers and buds. Based on our results with those obtained in previous research, we speculated that PbIDD2 and PbIDD8 might participate in flowering induction in pear. A temporal expression analysis showed that the expression patterns of PbIDD3 and PbIDD5 were completely opposite to the accumulation pattern of fruit lignin and the stone cell content. The results of the composite phylogenetic tree and expression pattern analysis indicated that PbIDD3 and PbIDD5 might be involved in the metabolism of lignin and secondary cell wall (SCW) formation. In summary, we provide basic information about the IDD genes in five Rosaceae species and thereby provide a theoretical basis for studying the function of these IDD genes.

Project description:The GATA gene family is one of the most important transcription factors (TFs). It extensively exists in plants, contributes to diverse biological processes such as the development process, and responds to environmental stress. Although the GATA gene family has been comprehensively and systematically studied in many species, less is known about GATA genes in Chinese pears (Pyrus bretschneideri). In the current study, the GATA gene family in the four Rosaceae genomes was identified, its structural characteristics identified, and a comparative analysis of its properties was carried out. Ninety-two encoded GATA proteins were authenticated in the four Rosaceae genomes (Pyrus bretschneideri, Prunus avium, Prunus mume, and Prunus persica) and categorized into four subfamilies (Ⅰ-Ⅳ) according to phylogeny. The majority of GATA genes contained one to two introns and conserved motif composition analysis revealed their functional divergence. Whole-genome duplications (WGDs) and dispersed duplication (DSD) played a key role in the expansion of the GATA gene family. The microarray indicated that, among P. bretschneideri, P. avium, P. mume and P. persica, GATA duplicated regions were more conserved between Pyrus bretschneideri and Prunus persica with 32 orthologous genes pairs. The physicochemical parameters, duplication patterns, non-synonymous (ka), and synonymous mutation rate (ks) and GO annotation ontology were performed using different bioinformatics tools. cis-elements respond to various phytohormones, abiotic/biotic stress, and light-responsive were found in the promoter regions of GATA genes which were induced via stimuli. Furthermore, subcellular localization of the PbGATA22 gene product was investigated, showing that it was present in the nucleus of tobacco (Nicotiana tabacum) epidermal cells. Finally, in silico analysis was performed on various organs (bud, leaf, stem, ovary, petal, and sepal) and different developmental stages of fruit. Subsequently, the expression profiles of PbGATA genes were extensively expressed under exogenous hormonal treatments of SA (salicylic acid), MeJA (methyl jasmonate), and ABA (abscisic acid) indicating that play important role in hormone signaling pathways. A comprehensive analysis of GATA transcription factors was performed through systematic biological approaches and comparative genomics to establish a theoretical base for further structural and functional investigations in Rosaceae species.

Dataset Information

Comparative genomic analysis of superoxide dismutase (SOD) genes in three Rosaceae species and expression analysis in Pyrus bretschneideri.

Supplementary information

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