Dataset Information

Kidney microRNA-21 Expression and Kidney Function in IgA Nephropathy.

ABSTRACT:

Rationale & objective

Previous studies have suggested that microRNA-21 (miR-21) plays an important role in kidney fibrosis. We examined the relationship between intrarenal miR-21 level and rate of kidney function loss in immunoglobulin A nephropathy (IgAN).

Study design

Prospective cohort study.

Setting & participants

40 patients with IgAN and 10 with hypertensive nephrosclerosis as controls.

Predictors

miR-21 levels in kidney biopsy specimen and urinary sediment, quantified as ratio to the housekeeping gene.

Outcomes

Kidney event-free survival and rate of kidney function decline.

Analytic approach

Time-to-event and correlation analysis.

Results

The IgAN group had significantly higher intrarenal miR-21 expression compared with the hypertensive nephrosclerosis group (1.71 [IQR, 0.99-2.77] vs 0.31 [IQR, 0.25-1.32]; P < 0.0001), but urinary miR-21 levels were similar. Intrarenal miR-21 expression had significant but modest correlation with severity of glomerulosclerosis (r = 0.293; P = 0.05) and tubulointerstitial fibrosis (r = 0.341; P = 0.03). Patients with high intrarenal miR-21 expression had significantly higher risk for developing kidney end points compared with those with low expression (log-rank test, P = 0.017). Univariate Cox analysis showed that intrarenal miR-21 expression significantly predicted the development of kidney end points (unadjusted HR, 1.586; 95% CI, 1.179-2.134; P = 0.002). However, the result was just short of statistical significance after adjusting for the severity of histologic damage (P = 0.06). There was also a significant correlation between intrarenal miR-21 expression and the slope of kidney function decline by univariate analysis (r = -0.399; P = 0.02).

Limitations

Small sample size; uncertain cellular origin of miR-21.

Conclusions

We found that intrarenal miR-21 expression is increased in patients with IgAN, modestly correlated with the severity of histologic damage, and predictive of subsequent kidney function loss.

SUBMITTER: Szeto CC

PROVIDER: S-EPMC7873829 | biostudies-literature | 2021 Jan-Feb

REPOSITORIES: biostudies-literature

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Publications

Kidney microRNA-21 Expression and Kidney Function in IgA Nephropathy.

Szeto Cheuk-Chun CC Ng Jack Kit-Chung JK Fung Winston Wing-Shing WW Luk Cathy Choi-Wan CC Wang Gang G Chow Kai-Ming KM Lai Ka-Bik KB Li Philip Kam-Tao PK Lai Fernand Mac-Moune FM

Kidney medicine 20201204 1

<h4>Rationale & objective</h4>Previous studies have suggested that microRNA-21 (miR-21) plays an important role in kidney fibrosis. We examined the relationship between intrarenal miR-21 level and rate of kidney function loss in immunoglobulin A nephropathy (IgAN).<h4>Study design</h4>Prospective cohort study.<h4>Setting & participants</h4>40 patients with IgAN and 10 with hypertensive nephrosclerosis as controls.<h4>Predictors</h4>miR-21 levels in kidney biopsy specimen and urinary sediment, qu ...[more]

PMID: 33604541

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Project description:IgA nephropathy (IgAN), the most frequent primary glomerulonephritis, affects young patients and is associated with a high risk of progression to end-stage renal disease. Consequently, patients with IgAN constitute an important proportion of candidates for kidney transplantation. Several studies showed a significant risk of IgAN recurrence on kidney graft, but the risks factors for recurrence remain to be accurately evaluated. Indeed, early identification of at risk patients may allow the optimization of treatment and the reduction of recurrence rate on the graft. In the present work, we studied the relationship between post-transplant serum IgA (sIgA) levels and the risk of IgAN recurrence after kidney transplantation. Recipients with IgAN had higher levels of sIgA as compared to patients with other nephropathies (p<0.05). The prevalence of IgAN recurrence was 20.8% during the period of analysis (mean follow-up of 6 ± 3.2 years). Serum IgA levels at M6, M12 and M24 post-transplant were significantly higher in patients with IgAN recurrence as compared to those without (p = 0.009, p = 0.035 and p = 0.029, respectively). Using receiver operating curve (ROC), sIgA at M6 and M12 post-transplant were significantly associated with IgAN recurrence (AUC = 0.771, p = 0.004 and AUC = 0.767, p = 0.016, respectively), while serum creatinine and proteinuria were not. Serum IgA level at month 6 was significantly associated with the occurrence of post-transplant IgA recurrence, whether it was analyzed as a continuous or a categorical variable. After successive adjustment on age, gender and proteinuria, sIgA remained a significant risk factor of post-transplant IgAN recurrence. Finally, survival free of IgAN recurrence was significantly better in patients with sIgA<222 mg/dL at month 6 as compare to IgAN patients with sIgA≥222 mg/dL (p = 0.03). Thus, the present work supports a link between post-transplant sIgA levels and IgAN recurrence and suggests that sIgA may be a valuable predictive biomarker of IgAN recurrence in kidney transplant recipients.

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Dataset Information

Kidney microRNA-21 Expression and Kidney Function in IgA Nephropathy.

Rationale & objective

Study design

Setting & participants

Predictors

Outcomes

Analytic approach

Results

Limitations

Conclusions

Publications

Kidney microRNA-21 Expression and Kidney Function in IgA Nephropathy.

Similar Datasets

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