Ontology highlight
ABSTRACT:
SUBMITTER: Ryan EL
PROVIDER: S-EPMC7875487 | biostudies-literature | 2021 Feb
REPOSITORIES: biostudies-literature
Ryan Ellis L EL Shelford James J Massam-Wu Teresa T Bayliss Richard R Royle Stephen J SJ
Journal of cell science 20210201 3
A multiprotein complex containing TACC3, clathrin and other proteins has been implicated in mitotic spindle stability. To disrupt this complex in an anti-cancer context, we need to understand its composition and how it interacts with microtubules. Induced relocalization of proteins in cells is a powerful way to analyze protein-protein interactions and, additionally, monitor where and when these interactions occur. We used CRISPR/Cas9 gene editing to add tandem FKBP-GFP tags to each complex membe ...[more]