Dataset Information

Effect of the Addition Frequency of 5-Azacytidine in Both Micro- and Macroscale Cultures.

ABSTRACT:

Introduction

Human mesenchymal stem cells (hMSCs) have a great clinical potential for tissue regeneration purposes due to its multilineage capability. Previous studies have reported that a single addition of 5-azacytidine (5-AzaC) causes the differentiation of hMSCs towards a myocardial lineage. The aim of this work was to evaluate the effect of 5-AzaC addition frequency on hMSCs priming (i.e., indicating an early genetic differentiation) using two culture environments.

Methods

hMSCs were supplemented with 5-AzaC while cultured in well plates and in microfluidic chips. The impact of 5-AzaC concentration (10 and 20 ?M) and addition frequency (once, daily or continuously), as well as of culture period (2 or 5 days) on the genetic upregulation of PPAR? (adipocytes), PAX3 (myoblasts), SOX9 (chondrocytes) and RUNX2 (osteoblasts) was evaluated.

Results

Daily delivering 5-AzaC caused a higher upregulation of PPAR?, SOX9 and RUNX2 in comparison to a single dose delivery, both under static well plates and dynamic microfluidic cultures. A particularly high gene expression of PPAR? (tenfold-change) could indicate priming of hMSCs towards adipocytes.

Conclusions

Both macro- and microscale cultures provided results with similar trends, where addition frequency of 5-AzaC was a crucial factor to upregulate several genes. Microfluidics technology was proven to be a suitable platform for the continuous delivery of a drug and could be used for screening purposes in tissue engineering research.

SUBMITTER: Kadekar S

PROVIDER: S-EPMC7878657 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature

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Publications

Effect of the Addition Frequency of 5-Azacytidine in Both Micro- and Macroscale Cultures.

Kadekar Sandeep S Barbe Laurent L Stoddart Martin M Varghese Oommen P OP Tenje Maria M Mestres Gemma G

Cellular and molecular bioengineering 20201006 1

<h4>Introduction</h4>Human mesenchymal stem cells (hMSCs) have a great clinical potential for tissue regeneration purposes due to its multilineage capability. Previous studies have reported that a single addition of 5-azacytidine (5-AzaC) causes the differentiation of hMSCs towards a myocardial lineage. The aim of this work was to evaluate the effect of 5-AzaC addition frequency on hMSCs priming (i.e., indicating an early genetic differentiation) using two culture environments.<h4>Methods</h4>hM ...[more]

PMID: 33633814

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Dataset Information

Effect of the Addition Frequency of 5-Azacytidine in Both Micro- and Macroscale Cultures.

Introduction

Methods

Results

Conclusions

Publications

Effect of the Addition Frequency of 5-Azacytidine in Both Micro- and Macroscale Cultures.

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