Ontology highlight
ABSTRACT:
SUBMITTER: Aupic J
PROVIDER: S-EPMC7878764 | biostudies-literature | 2021 Feb
REPOSITORIES: biostudies-literature
Aupič Jana J Strmšek Žiga Ž Lapenta Fabio F Pahovnik David D Pisanski Tomaž T Drobnak Igor I Ljubetič Ajasja A Jerala Roman R
Nature communications 20210211 1
Natural proteins are characterised by a complex folding pathway defined uniquely for each fold. Designed coiled-coil protein origami (CCPO) cages are distinct from natural compact proteins, since their fold is prescribed by discrete long-range interactions between orthogonal pairwise-interacting coiled-coil (CC) modules within a single polypeptide chain. Here, we demonstrate that CCPO proteins fold in a stepwise sequential pathway. Molecular dynamics simulations and stopped-flow Förster resonanc ...[more]