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Three-dimensional vascular microenvironment landscape in human glioblastoma.


ABSTRACT: The cellular complexity of glioblastoma microenvironments is still poorly understood. In-depth, cell-resolution tissue analyses of human material are rare but highly necessary to understand the biology of this deadly tumor. Here we present a unique 3D visualization revealing the cellular composition of human GBM in detail and considering its critical association with the neo-vascular niche. Our images show a complex vascular map of human 3D biopsies with increased vascular heterogeneity and altered spatial relationship with astrocytes or glioma-cell counterparts. High-resolution analysis of the structural layers of the blood brain barrier showed a multilayered fenestration of endothelium and basement membrane. Careful examination of T cell position and migration relative to vascular walls revealed increased infiltration corresponding with tumor proliferation. In addition, the analysis of the myeloid landscape not only showed a volumetric increase in glioma-associated microglia and macrophages relative to GBM proliferation but also revealed distinct phenotypes in tumor nest and stroma. Images and data sets are available on demand as a resource for public access.

SUBMITTER: Cribaro GP 

PROVIDER: S-EPMC7879533 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature

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Three-dimensional vascular microenvironment landscape in human glioblastoma.

Cribaro George P GP   Saavedra-López Elena E   Romarate Leire L   Mitxitorena Izaskun I   Díaz Laura R LR   Casanova Paola V PV   Roig-Martínez Meritxell M   Gallego José M JM   Perez-Vallés Ana A   Barcia Carlos C  

Acta neuropathologica communications 20210212 1


The cellular complexity of glioblastoma microenvironments is still poorly understood. In-depth, cell-resolution tissue analyses of human material are rare but highly necessary to understand the biology of this deadly tumor. Here we present a unique 3D visualization revealing the cellular composition of human GBM in detail and considering its critical association with the neo-vascular niche. Our images show a complex vascular map of human 3D biopsies with increased vascular heterogeneity and alte  ...[more]

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