Dataset Information

The relationship between abnormal Core binding factor-? expression in human cartilage and osteoarthritis.

ABSTRACT:

Background

This study aimed to investigate the effect of abnormal Core binding factor-? expression on proliferation, differentiation and apoptosis of chondrocytes, and elucidate the relationship between Core binding factor-? and osteoarthritis-related markers and degenerative joint disease.

Methods

Cartilage tissues, from healthy subjects and patients with osteoarthritis, were collected for histology and expression of Core binding factor-?, MMP-13, IL-1?, COMP, and YKL-40. Human articular chondrocytes were cultured in vitro, and a viral vector was constructed to regulate cellular Core binding factor-? expression. Cellular proliferation and apoptosis were observed, and osteoarthritis-related inflammatory factor expression and cartilage metabolite synthesis assayed.

Results

Human osteoarthritis lesions had disordered cartilage structure and cellular arrangement, and increased emptying of cartilage lacunae. Normal cell counts were significantly reduced, cartilage extracellular matrix was obviously damaged, and type II collagen expression was significantly decreased. Core binding factor-? was highly expressed in the osteoarthritis cartilage (p?ConclusionsAbnormal Core binding factor-? expression might play an upstream regulatory role in mediating abnormal chondrocyte apoptosis and the inflammatory response. On inhibiting Core binding factor-? expression, a delay in cartilage degeneration was expected.

Trial registration

The study was registered for clinical trials in ChiCTR: ChiCTR1800017066 (Reg. Date-2018/7/10).

SUBMITTER: Li G

PROVIDER: S-EPMC7879671 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature

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Publications

The relationship between abnormal Core binding factor-β expression in human cartilage and osteoarthritis.

Li Guangdi G Zhang Mi M Huang Yuan Y Yang Jiafei J Dong Lianghong L Shi Hao H Li Long L Liu Riguang R Li Jiangwei J

BMC musculoskeletal disorders 20210211 1

<h4>Background</h4>This study aimed to investigate the effect of abnormal Core binding factor-β expression on proliferation, differentiation and apoptosis of chondrocytes, and elucidate the relationship between Core binding factor-β and osteoarthritis-related markers and degenerative joint disease.<h4>Methods</h4>Cartilage tissues, from healthy subjects and patients with osteoarthritis, were collected for histology and expression of Core binding factor-β, MMP-13, IL-1β, COMP, and YKL-40. Human a ...[more]

PMID: 33573620

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