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Generation of inner ear sensory neurons using blastocyst complementation in a Neurog1^+/--deficient mouse.

ABSTRACT: This research is the first to produce induced pluripotent stem cell-derived inner ear sensory neurons in the Neurog1^+/- heterozygote mouse using blastocyst complementation. Additionally, this approach corrected non-sensory deficits associated with Neurog1 heterozygosity, indicating that complementation is specific to endogenous Neurog1 function. This work validates the use of blastocyst complementation as a tool to create novel insight into the function of developmental genes and highlights blastocyst complementation as a potential platform for generating chimeric inner ear cell types that can be transplanted into damaged inner ears to improve hearing.

SUBMITTER: Steevens AR

PROVIDER: S-EPMC7881691 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature

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Generation of inner ear sensory neurons using blastocyst complementation in a Neurog1<sup>+/-</sup>-deficient mouse.

Steevens Aleta R AR Griesbach Matthew W MW You Yun Y Dutton James R JR Low Walter C WC Santi Peter A PA

Stem cell research & therapy 20210212 1

This research is the first to produce induced pluripotent stem cell-derived inner ear sensory neurons in the Neurog1<sup>+/-</sup> heterozygote mouse using blastocyst complementation. Additionally, this approach corrected non-sensory deficits associated with Neurog1 heterozygosity, indicating that complementation is specific to endogenous Neurog1 function. This work validates the use of blastocyst complementation as a tool to create novel insight into the function of developmental genes and high ...[more]

PMID: 33579352

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