Distinguishing SARS-CoV-2 bonafide re-infection from pre-existing minor variant reactivation.

ABSTRACT: Different groups have recently reported events of SARS-CoV-2 reinfection, where patients had a sequence of positive-negative-positive RT-PCR tests. However, such events could be explained by different scenarios such as intermittent viral shedding, bonafide re-infection or multiple infection with alternating predominance of different viruses. Analysis of minor variants is an important tool to distinguish between these scenarios. Using ARTIC network PCR amplification and next-generation sequencing, we obtained SARS-CoV-2 sequences from two timepoints (with a time span of 102 days) of a patient followed at the Brazilian National Cancer Institute. Within-host variant analysis evidenced three single nucleotide variants (SNVs) at the consensus viral sequence in the second timepoint that were alr

SUBMITTER: Siqueira JD 

PROVIDER: S-EPMC7882217 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature