Comparison of BCYEα+AB agar and MWY agar for detection and enumeration of Legionella spp. in hospital water samples.

ABSTRACT:

Background

This study illustrates for the first time the performance (sensitivity and selectivity) of the selective medium BCYEα +AB suggested by the new edition of ISO 11731 for legionella isolation and enumeration. We compared the efficacy of the selective BCYEα +AB medium with that of the highly selective MWY medium.

Results

Legionella spp. was detected in 48.2 and 47.1% of the samples by BCYEα +AB and MWY agar, respectively. For optimal detection of Legionella spp., most protocols recommend using selective media to reduce the number of non-Legionella bacteria. Agreement between the two media was 86.7%.

Conclusions

According to the results, both media have a very similar performance and they both have advantages and disadvantages over each other. In AB medium there

SUBMITTER: Ditommaso S 

PROVIDER: S-EPMC7885575 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature