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The methionase chain reaction: an enzyme-based autocatalytic amplification system for the detection of thiols.

ABSTRACT: We present an autocatalytic system for the detection and amplification of thiols termed the Methionase Chain Reaction (MCR). MCR is based on the reversible modification of the thiol producing enzyme Methionine Gamma-Lyase (MGL). MCR was able to amplify the concentration of thiols by a factor of 560 and was able to visually detect thiols at concentrations as low as 50 nM.

SUBMITTER: Adams JD

PROVIDER: S-EPMC7886004 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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The methionase chain reaction: an enzyme-based autocatalytic amplification system for the detection of thiols.

Adams Jeremy David JD Røise Joachim Justad JJ Lee David Sam DS Murthy Niren N

Chemical communications (Cambridge, England) 20200217 21

We present an autocatalytic system for the detection and amplification of thiols termed the Methionase Chain Reaction (MCR). MCR is based on the reversible modification of the thiol producing enzyme Methionine Gamma-Lyase (MGL). MCR was able to amplify the concentration of thiols by a factor of 560 and was able to visually detect thiols at concentrations as low as 50 nM. ...[more]

PMID: 32065188

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