Molecular Imaging of Chimeric Antigen Receptor T Cells by ICOS-ImmunoPET.

ABSTRACT:

Purpose

Immunomonitoring of chimeric antigen receptor (CAR) T cells relies primarily on their quantification in the peripheral blood, which inadequately quantifies their biodistribution and activation status in the tissues. Noninvasive molecular imaging of CAR T cells by PET is a promising approach with the ability to provide spatial, temporal, and functional information. Reported strategies rely on the incorporation of reporter transgenes or ex vivo biolabeling, significantly limiting the application of CAR T-cell molecular imaging. In this study, we assessed the ability of antibody-based PET (immunoPET) to noninvasively visualize CAR T cells.

Experimental design

After analyzing human CAR T cells in vitro and ex vivo from patient samples to identify can

SUBMITTER: Simonetta F 

PROVIDER: S-EPMC7887027 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature