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The efficacy data of two household cleaning and disinfecting agents on Lens culinaris Medik and Vicia faba seed germination.

ABSTRACT: A germination test of Vicia faba and Lens culinaris seeds under the effect of bleach and vinegar was conducted for seven days, and the observations were recorded daily. The completely randomized design (CRD) was used to examine the germination with three replicates at the lab conditions. Six germination parameters were measured, including germination percentage (GP), germination index (GI), mean germination time (MGT), mean germination rate (MGR), vigour index (VI), plus the fresh weight (FW) and dry weight (DW) of Vicia faba and Lens culinaris seeds. As a legume crop seeds model, the efficacy of four treatment levels from 0.005% to 0.5% of bleach and vinegar on the germination was tested. A chemical analysis was performed using the ion chromatography (IC) to evaluate the effect of chloride and acetate anions uptake on the seedling germination in addition to other essential nutrients. A significant inhibition in seedling growth was observed with increasing the treatment concentration. The maximum inhibition was recorded for both seeds at 0.5%, followed by 0.1% levels, while a positive effect was represented with the lower concentrations. The chemical analysis of the up taking active ingredients was corroborated the germination outputs.

SUBMITTER: Elezz AA

PROVIDER: S-EPMC7887392 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature

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The efficacy data of two household cleaning and disinfecting agents on Lens culinaris Medik and Vicia faba seed germination.

Elezz Ahmed Abou AA Ahmed Talaat T

Data in brief 20210129

A germination test of Vicia faba and Lens culinaris seeds under the effect of bleach and vinegar was conducted for seven days, and the observations were recorded daily. The completely randomized design (CRD) was used to examine the germination with three replicates at the lab conditions. Six germination parameters were measured, including germination percentage (GP), germination index (GI), mean germination time (MGT), mean germination rate (MGR), vigour index (VI), plus the fresh ...[more]

PMID: 33644269

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Project description:BackgroundThe development of genetic markers is complex and costly in species with little pre-existing genomic information. Faba bean possesses one of the largest and least studied genomes among cultivated crop plants and no gene-based genetic maps exist. Gene-based orthologous markers allow chromosomal regions and levels of synteny to be characterised between species, reveal phylogenetic relationships and chromosomal evolution, and enable targeted identification of markers for crop breeding. In this study orthologous codominant cross-species markers have been deployed to produce the first exclusively gene-based genetic linkage map of faba bean (Vicia faba), using an F6 population developed from a cross between the lines Vf6 (equina type) and Vf27 (paucijuga type).ResultsOf 796 intron-targeted amplified polymorphic (ITAP) markers screened, 151 markers could be used to construct a comparative genetic map. Linkage analysis revealed seven major and five small linkage groups (LGs), one pair and 12 unlinked markers. Each LG was comprised of three to 30 markers and varied in length from 23.6 cM to 324.8 cM. The map spanned a total length of 1685.8 cM. A simple and direct macrosyntenic relationship between faba bean and Medicago truncatula was evident, while faba bean and lentil shared a common rearrangement relative to M. truncatula. One hundred and four of the 127 mapped markers in the 12 LGs, which were previously assigned to M. truncatula genetic and physical maps, were found in regions syntenic between the faba bean and M. truncatula genomes. However chromosomal rearrangements were observed that could explain the difference in chromosome numbers between these three legume species. These rearrangements suggested high conservation of M. truncatula chromosomes 1, 5 and 8; moderate conservation of chromosomes 2, 3, 4 and 7 and no conservation with M. truncatula chromosome 6. Multiple PCR amplicons and comparative mapping were suggestive of small-scale duplication events in faba bean. This study also provides a preliminary indication for finer scale macrosynteny between M. truncatula, lentil and faba bean. Markers originally designed from genes on the same M. truncatula BACs were found to be grouped together in corresponding syntenic areas in lentil and faba bean.ConclusionDespite the large size of the faba bean genome, comparative mapping did not reveal evidence for polyploidisation, segmental duplication, or significant rearrangements compared to M. truncatula, although a bias in the use of single locus markers may have limited the detection of duplications. Non-coding repetitive DNA or transposable element content provides a possible explanation for the difference in genome sizes. Similar patterns of rearrangements in faba bean and lentil compared to M. truncatula support phylogenetic studies dividing these species into the tribes Viceae and Trifoliae. However, substantial macrosynteny was apparent between faba bean and M. truncatula, with the exception of chromosome 6 where no orthologous markers were found, confirming previous investigations suggesting chromosome 6 is atypical. The composite map, anchored with orthologous markers mapped in M. truncatula, provides a central reference map for future use of genomic and genetic information in faba bean genetic analysis and breeding.

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Dataset Information

The efficacy data of two household cleaning and disinfecting agents on Lens culinaris Medik and Vicia faba seed germination.

Publications

The efficacy data of two household cleaning and disinfecting agents on <i>Lens culinaris Medik</i> and <i>Vicia faba</i> seed germination.

Similar Datasets

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