Plasma-mediated enhancement of enzyme secretion in Aspergillus oryzae.

ABSTRACT: Technical bottlenecks in protein production and secretion often limit the efficient and robust industrial use of microbial enzymes. The potential of non-thermal atmospheric pressure plasma to overcome these technical barriers was examined. Spores of the fermenting fungus Aspergillus oryzae (A. oryzae) were submerged in potato dextrose broth (PDB) (5 × 10⁶ per ml) and treated with micro dielectric barrier discharge plasma at an input voltage of 1.2 kV and current of 50 to 63 mA using nitrogen as the feed gas. The specific activity of α-amylase in the broth was increased by 7.4 to 9.3% after 24 and 48 h of plasma treatment. Long-lived species, such as NO₂ ^- and NO₃ ^- , generated in PDB after plasma treatment may have contributed to the e

SUBMITTER: Veerana M 

PROVIDER: S-EPMC7888467 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature