Dataset Information

Characterization of the complete chloroplast genome of 'Yunning No.1' lemon (Citrus limon).

ABSTRACT: 'Yunning No.1' lemon, a mutant of Eureka lemon, is originally found in Yunnan province of China and is the main cultivated lemon variety there. In this study, we assembled and annotated its chloroplast genome using Illumina Hiseq-2500 whole genome re-sequencing data. Its chloroplast genome is 160,141 bp in size, containing a 87,754 bp large single copy region, a 18,385 bp small single copy region and a pair of 27,001 bp inverted repeat region. Like many citrus species, 114 unique genes (including 80 protein-coding genes, 30 tRNAs and 4 rRNAs) could be identified from the chloroplast genome of 'Yunning No.1'. Phylogenetic analysis revealed that the 'Yunning No.1' chloroplast genome was closest to Citrus maxima.

SUBMITTER: Wang SH

PROVIDER: S-EPMC7889090 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature

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Characterization of the complete chloroplast genome of 'Yunning No.1' lemon (<i>Citrus limon</i>).

Wang Shao-Hua SH Deng Guang-Zhou GZ Gao Jun-Yan JY Liu Hong-Ming HM Zhou Xian-Yan XY Yue Jian-Qiang JQ Dong Mei-Chao MC Yang Fan F Zhou Dong-Guo DG Guo Li-Na LN Yuan Xiao-Hui XH Long Chun-Rui CR

Mitochondrial DNA. Part B, Resources 20210209 2

'Yunning No.1' lemon, a mutant of Eureka lemon, is originally found in Yunnan province of China and is the main cultivated lemon variety there. In this study, we assembled and annotated its chloroplast genome using Illumina Hiseq-2500 whole genome re-sequencing data. Its chloroplast genome is 160,141 bp in size, containing a 87,754 bp large single copy region, a 18,385 bp small single copy region and a pair of 27,001 bp inverted repeat region. Like many citrus species, 114 unique genes (includin ...[more]

PMID: 33628878

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Project description:BackgroundFlavonoids have shown to exert multiple beneficial effects on human health, being also appreciated by both food and pharmaceutical industries. Citrus fruits are a key source of flavonoids, thus promoting studies to obtain them. Characteristics of these studies are the discrepancies among sample pretreatments and among extraction methods, and also the scant number of comparative studies developed so far.ObjectiveEvaluate the effect of both the sample pretreatment and the extraction method on the profile of flavonoids isolated from lemon.ResultsExtracts from fresh, lyophilized and air-dried samples obtained by shaking extraction (SE), ultrasound-assisted extraction (USAE), microwave-assisted extraction (MAE) and superheated liquid extraction (SHLE) were analyzed by LC-QTOF MS/MS, and 32 flavonoids were tentatively identified using MS/MS information. ANOVA applied to the data from fresh and dehydrated samples and from extraction by the different methods revealed that 26 and 32 flavonoids, respectively, were significant (p≤0.01). The pairwise comparison (Tukey HSD; p≤0.01) showed that lyophilized samples are more different from fresh samples than from air-dried samples; also, principal component analysis (PCA) showed a clear discrimination among sample pretreatment strategies and suggested that such differences are mainly created by the abundance of major flavonoids. On the other hand, pairwise comparison of extraction methods revealed that USAE and MAE provided quite similar extracts, being SHLE extracts different from the other two. In this case, PCA showed a clear discrimination among extraction methods, and their position in the scores plot suggests a lower abundance of flavonoids in the extracts from SHLE. In the two PCA the loadings plots revealed a trend to forming groups according to flavonoid aglycones.ConclusionsThe present study shows clear discrimination caused by both sample pretreatments and extraction methods. Under the studied conditions, liophilization provides extracts with higher amounts of flavonoids, and USAE is the best method for isolation of these compounds, followed by MAE and SE. On the contrary, the SHLE method was the less favorable to extract flavonoids from citrus owing to degradation.

Dataset Information

Characterization of the complete chloroplast genome of 'Yunning No.1' lemon (Citrus limon).

Publications

Characterization of the complete chloroplast genome of 'Yunning No.1' lemon (<i>Citrus limon</i>).

Similar Datasets

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