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A novel endogenous CD16-Expressing Natural Killer Cell for cancer immunotherapy.

ABSTRACT: Natural killer (NK) cells, as a potential source for off-the-shelf cell therapy, attack tumor cells with low risk of severe cytokine release syndrome (CRS) or graft-versus-host disease (GvHD). Fc? receptor IIIA, also known as CD16, further confers NK cells with antibody-dependent cell-mediated cytotoxicity (ADCC), one mechanism of action of antibody-based immunotherapy. Here, we establish a novel human NK cell line, oNK-1, endogenously expressing CD16 along with high levels of NK activation markers and low levels of NK inhibitory markers. The long-term expansion and CD16 expression of oNK-1 cells were demonstrated. Furthermore, oNK-1 cells elicit superior cytotoxicity against cancer cells than primary NK cells. In conclusion, this study suggests that endogenous CD16-expressing oNK-1 has the potential to develop an effective NK-based therapy.

SUBMITTER: Cheng ZF

PROVIDER: S-EPMC7889824 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature

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A novel endogenous CD16-Expressing Natural Killer Cell for cancer immunotherapy.

Cheng Zih-Fei ZF Li Hao-Kang HK Yang Hsiu-Ping HP Lee Chia-Yun CY Tang Sai-Wen SW Lin Yan-Liang YL Hsiao Shih-Chia SC

Biochemistry and biophysics reports 20210203

Natural killer (NK) cells, as a potential source for off-the-shelf cell therapy, attack tumor cells with low risk of severe cytokine release syndrome (CRS) or graft-versus-host disease (GvHD). Fcγ receptor IIIA, also known as CD16, further confers NK cells with antibody-dependent cell-mediated cytotoxicity (ADCC), one mechanism of action of antibody-based immunotherapy. Here, we establish a novel human NK cell line, oNK-1, endogenously expressing CD16 along with high levels of NK activation mark ...[more]

PMID: 33644421

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