Dataset Information

Mechanical stretch induces Ca²⁺ influx and extracellular release of PGE₂ through Piezo1 activation in trabecular meshwork cells.

ABSTRACT: The trabecular meshwork (TM) constitutes the main pathway for aqueous humor drainage and is exposed to complex intraocular pressure fluctuations. The mechanism of homeostasis in which TM senses changes in intraocular pressure and leads to normal levels of outflow resistance is not yet well understood. Previous reports have shown that Piezo1, a mechanically-activated cation channel, is expressed in TM and isolated TM cells. Therefore, we tested hypothesis that Piezo1 may function in response to membrane tension and stretch in TM. In human trabecular meshwork (hTM) cells, PIEZO1 was showed to be abundantly expressed, and Piezo1 agonist Yoda1 and mechanical stretch caused a Piezo1-dependent Ca²⁺ influx and release of arachidonic acid and PGE₂. Treatment with Yoda1 or PGE₂ significantly inhibited hTM cell contraction. These results suggest that mechanical stretch stimuli in TM activates Piezo1 and subsequently regulates TM cell contraction by triggering Ca²⁺ influx and release of arachidonic acid and PGE₂. Thus, Piezo1 could acts as a regulator of intraocular pressure (IOP) within the conventional outflow pathway and could be a novel therapeutic strategy to modulate IOP in glaucoma patients.

SUBMITTER: Uchida T

PROVIDER: S-EPMC7890064 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature

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Mechanical stretch induces Ca<sup>2+</sup> influx and extracellular release of PGE<sub>2</sub> through Piezo1 activation in trabecular meshwork cells.

Uchida Takatoshi T Shimizu Shota S Yamagishi Reiko R Tokuoka Suzumi M SM Kita Yoshihiro Y Honjo Megumi M Aihara Makoto M

Scientific reports 20210217 1

The trabecular meshwork (TM) constitutes the main pathway for aqueous humor drainage and is exposed to complex intraocular pressure fluctuations. The mechanism of homeostasis in which TM senses changes in intraocular pressure and leads to normal levels of outflow resistance is not yet well understood. Previous reports have shown that Piezo1, a mechanically-activated cation channel, is expressed in TM and isolated TM cells. Therefore, we tested hypothesis that Piezo1 may function in response to m ...[more]

PMID: 33597646

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Project description:Key pointsTrabecular meshwork (TM) is a highly mechanosensitive tissue in the eye that regulates intraocular pressure through the control of aqueous humour drainage. Its dysfunction underlies the progression of glaucoma but neither the mechanisms through which TM cells sense pressure nor their role in aqueous humour outflow are understood at the molecular level. We identified the Piezo1 channel as a key TM transducer of tensile stretch, shear flow and pressure. Its activation resulted in intracellular signals that altered organization of the cytoskeleton and cell-extracellular matrix contacts and modulated the trabecular component of aqueous outflow whereas another channel, TRPV4, mediated a delayed mechanoresponse. This study helps elucidate basic mechanotransduction properties that may contribute to intraocular pressure regulation in the vertebrate eye.AbstractChronic elevations in intraocular pressure (IOP) can cause blindness by compromising the function of trabecular meshwork (TM) cells in the anterior eye, but how these cells sense and transduce pressure stimuli is poorly understood. Here, we demonstrate functional expression of two mechanically activated channels in human TM cells. Pressure-induced cell stretch evoked a rapid increase in transmembrane current that was inhibited by antagonists of the mechanogated channel Piezo1, Ruthenium Red and GsMTx4, and attenuated in Piezo1-deficient cells. The majority of TM cells exhibited a delayed stretch-activated current that was mediated independently of Piezo1 by TRPV4 (transient receptor potential cation channel, subfamily V, member 4) channels. Piezo1 functions as the principal TM transducer of physiological levels of shear stress, with both shear and the Piezo1 agonist Yoda1 increasing the number of focal cell-matrix contacts. Analysis of TM-dependent fluid drainage from the anterior eye showed significant inhibition by GsMTx4. Collectively, these results suggest that TM mechanosensitivity utilizes kinetically, regulatory and functionally distinct pressure transducers to inform the cells about force-sensing contexts. Piezo1-dependent control of shear flow sensing, calcium homeostasis, cytoskeletal dynamics and pressure-dependent outflow suggests potential for a novel therapeutic target in treating glaucoma.

Dataset Information

Mechanical stretch induces Ca²⁺ influx and extracellular release of PGE₂ through Piezo1 activation in trabecular meshwork cells.

Publications

Mechanical stretch induces Ca<sup>2+</sup> influx and extracellular release of PGE<sub>2</sub> through Piezo1 activation in trabecular meshwork cells.

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Dataset Information

Mechanical stretch induces Ca2+ influx and extracellular release of PGE2 through Piezo1 activation in trabecular meshwork cells.

Publications

Mechanical stretch induces Ca<sup>2+</sup> influx and extracellular release of PGE<sub>2</sub> through Piezo1 activation in trabecular meshwork cells.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Mechanical stretch induces Ca²⁺ influx and extracellular release of PGE₂ through Piezo1 activation in trabecular meshwork cells.