Dataset Information

Characterizing the putative mitogen-activated protein kinase (MAPK) and their protective role in oxidative stress tolerance and carbon assimilation in wheat under terminal heat stress.

ABSTRACT: Wheat, being sensitive to terminal heat, causes drastic reduction in grain quality and yield. MAPK cascade regulates the network of defense mechanism operated inside plant system. Here, we have identified 21 novel MAPKs through gel-based proteomics and RNA-seq data analysis. Based on digital gene expression, two transcripts (transcript_2834 and transcript_8242) showing homology with MAPK were cloned and characterized from wheat (acc. nos. MK854806 and KT835664). Transcript_2834 was cloned in pET28a vector and recombinant MAPK protein of ?40.3 kDa was isolated and characterized to have very high in-vitro kinase activity under HS. Native MAPK showed positive correlation with the expression of TFs, HSPs, genes linked with antioxidant enzyme (SOD, CAT, GPX), photosynthesis and starch biosynthesis pathways in wheat under HS. Wheat cv. HD3086 (thermotolerant) having higher expression and activity of MAPK under HS showed significant increase in accumulation of proline, H₂O₂, starch, and granule integrity, compared with BT-Schomburgk (thermosusceptible).

SUBMITTER: Kumar RR

PROVIDER: S-EPMC7890154 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature

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Characterizing the putative mitogen-activated protein kinase (<i>MAPK</i>) and their protective role in oxidative stress tolerance and carbon assimilation in wheat under terminal heat stress.

Kumar Ranjeet R RR Dubey Kavita K Arora Kirti K Dalal Monika M Rai Gyanendra K GK Mishra Dwijesh D Chaturvedi Krishna K KK Rai Anil A Kumar Soora Naresh SN Singh Bhupinder B Chinnusamy Viswanathan V Praveen Shelly S

Biotechnology reports (Amsterdam, Netherlands) 20210205

Wheat, being sensitive to terminal heat, causes drastic reduction in grain quality and yield. MAPK cascade regulates the network of defense mechanism operated inside plant system. Here, we have identified 21 novel MAPKs through gel-based proteomics and RNA-seq data analysis. Based on digital gene expression, two transcripts (transcript_2834 and transcript_8242) showing homology with MAPK were cloned and characterized from wheat (acc. nos. MK854806 and KT835664). Transcript_2834 was cloned in pET ...[more]

PMID: 33659194

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Project description:Terminal heat stress has become one of the major threats due to global climate change which is significantly affecting the production and productivity of wheat crop. Therefore, it is necessary to identify key traits and genotypes to breed heat-tolerant wheat. The present study was undertaken with the objective of comparing the effects of heat stress (HSE) and extended heat stress (EHSE) on phenological-physio-biochemical traits of contrasting heat-tolerant and heat-susceptible genotypes during the reproductive phase. Phenological traits exhibited significant reduction under EHSE compared to HSE. Heat-tolerant genotypes maintained balanced phenological-physio-biochemical traits, while heat-sensitive genotypes showed significant reductions under both stress regimes. Among phenological traits, DM (R2 = 0.52) and BY (R2 = 0.44) have shown a positive effect on seed yield, indicating that biomass and crop duration contributed to the yield advantage under stress. During the grain filling stage, both the normalized difference vegetation index (NDVI) and chlorophyll (Chl) exhibited consistently positive impacts on grain yield under both HSE and EHSE conditions. This could be attributed to the enhanced photosynthesis resulting from delayed senescence and improved assimilate remobilization under terminal heat stress. The biochemical activity of superoxide dismutase (SOD), peroxidase (POX), and ascorbate peroxidase (APX) was induced in tolerant genotypes under HSE. The correlation of canopy temperature with phenological-physio-biochemical traits remained static under HSE and EHSE, suggesting CT as the best selection parameter for heat tolerance. The traits showing a positive association with yield and that are less affected under stress could be used for selecting tolerant genotypes under stress environments. These tolerant genotypes can be used to develop mapping populations to decipher the genes conferring tolerance as well as to study the molecular basis of tolerance.

Dataset Information

Characterizing the putative mitogen-activated protein kinase (MAPK) and their protective role in oxidative stress tolerance and carbon assimilation in wheat under terminal heat stress.

Publications

Characterizing the putative mitogen-activated protein kinase (<i>MAPK</i>) and their protective role in oxidative stress tolerance and carbon assimilation in wheat under terminal heat stress.

Similar Datasets

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