Dataset Information

Transcriptional activity of vitamin D receptor in human periodontal ligament cells is diminished under inflammatory conditions.

ABSTRACT:

Background

Although vitamin D₃ deficiency is considered as a risk factor for periodontitis, supplementation during periodontal treatment has not been shown to be beneficial to date. Human periodontal ligament cells (hPDLCs) are regulated by vitamin D₃ and play a fundamental role in periodontal tissue homeostasis and inflammatory response in periodontitis. The aim of this study is to investigate possible alterations of the vitamin D₃ activity in hPDLCs under inflammatory conditions.

Methods

Cells isolated from six different donors were treated with either 1,25(OH)₂ D₃ (0 to 10 nM) or 25(OH)D₃ (0 to 100 nM) in the presence and absence of ultrapure or standard Porphyromonas gingivalis lipopolysaccharide (PgLPS), Pam3CSK4, or interferon-? for 48 hours. Additionally, nuclear factor (NF)-?B inhibition was performed with BAY 11-7082. The bioactivity of vitamin D in hPDLCs was assessed based on the gene expression levels of vitamin D receptor (VDR)-regulated genes osteocalcin and osteopontin. Additionally, VDR and CYP27B1 expression levels were measured.

Results

The vitamin D₃ -induced increase of osteocalcin and osteopontin expression was significantly decreased in the presence of standard PgLPS and Pam3CSK4, which was not observed by ultrapure PgLPS. Interferon-y had diverse effects on the response of hPDLCs to vitamin D₃ metabolites. NF-kB inhibition abolished the effects of standard PgLPS and Pam3CSK4. Standard PgLPS and Pam3CSK4 increased VDR expression in the presence of vitamin D₃ . CYP27B1 expression was not affected by vitamin D₃ and inflammatory conditions.

Conclusions

This study indicates that the transcriptional activity of VDR is diminished under inflammatory conditions, which might mitigate the effectiveness of vitamin D₃ supplementation during periodontal treatment.

SUBMITTER: Blufstein A

PROVIDER: S-EPMC7891446 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

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Publications

Transcriptional activity of vitamin D receptor in human periodontal ligament cells is diminished under inflammatory conditions.

Blufstein Alice A Behm Christian C Kubin Barbara B Gahn Johannes J Moritz Andreas A Rausch-Fan Xiaohui X Andrukhov Oleh O

Journal of periodontology 20200621 1

<h4>Background</h4>Although vitamin D<sub>3</sub> deficiency is considered as a risk factor for periodontitis, supplementation during periodontal treatment has not been shown to be beneficial to date. Human periodontal ligament cells (hPDLCs) are regulated by vitamin D<sub>3</sub> and play a fundamental role in periodontal tissue homeostasis and inflammatory response in periodontitis. The aim of this study is to investigate possible alterations of the vitamin D<sub>3</sub> activity in hPDLCs und ...[more]

PMID: 32474936

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Dataset Information

Transcriptional activity of vitamin D receptor in human periodontal ligament cells is diminished under inflammatory conditions.

Background

Methods

Results

Conclusions

Publications

Transcriptional activity of vitamin D receptor in human periodontal ligament cells is diminished under inflammatory conditions.

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