Dataset Information

Histomorphological investigation of intrahepatic connective tissue for surgical anatomy based on modern computer imaging analysis.

ABSTRACT:

Background/purpose

Computer-assisted tissue imaging and analytical techniques were used to clarify the histomorphological structure of hepatic connective tissue as a practical guide for surgeons.

Methods

Approximately 5000 histological slides were prepared from liver specimens of five autopsied patients. Three-dimensional (3D) reconstruction was performed and subjected to computer imaging analysis. Scanning electron microscopy was also performed on the liver specimens.

Results

The 3D reconstructed images revealed the running form of the vasculature and the relationship between the hepatic lobule and connective tissue. The hepatic capsule or portal pedicle was consistently located at the periphery of the hepatic lobules. An artificial intelligence random forest approach clearly segmented hepatic cells, type I collagen (CF), type III collagen (RF), and other cells. The hepatic lobule, portal region, and hepatic capsule were significantly distinguished based on CF and RF occupancy. The capsule directly covering the liver lobule with an RF concentration up to 87% was provisionally named the proper hepatic capsule. The existence of a proper hepatic ligament with distinct occupation rates of CF and RF was also suggested.

Conclusions

The identified proper hepatic capsule and ligament can be important markers for demarcating the dissecting layer during surgical procedures.

SUBMITTER: Ikeda T

PROVIDER: S-EPMC7891672 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Histomorphological investigation of intrahepatic connective tissue for surgical anatomy based on modern computer imaging analysis.

Ikeda Tetsuo T Okano Shinji S Hashimoto Naotaka N Kimura Koichi K Kudo Kensuke K Tsutsumi Ryosuke R Sasaki Shun S Kawasaki Junji J Miyashita Yu Y Wada Hiroya H

Journal of hepato-biliary-pancreatic sciences 20200722 1

<h4>Background/purpose</h4>Computer-assisted tissue imaging and analytical techniques were used to clarify the histomorphological structure of hepatic connective tissue as a practical guide for surgeons.<h4>Methods</h4>Approximately 5000 histological slides were prepared from liver specimens of five autopsied patients. Three-dimensional (3D) reconstruction was performed and subjected to computer imaging analysis. Scanning electron microscopy was also performed on the liver specimens.<h4>Results< ...[more]

PMID: 32697892

Similar Datasets

Project description:In this review, we highlight the complexities of the natural history, biology, and clinical management of three intermediate connective tissue tumors: desmoid tumor (DT) or aggressive fibromatosis, tenosynovial giant cell tumor (TGCT) or diffuse-type pigmented villonodular synovitis (dtPVNS), and giant cell tumor of bone (GCTB). Intermediate histologies include tumors of both soft tissue and bone origin and are locally aggressive and rarely metastatic. Some common aspects to these tumors are that they can be locally infiltrative and/or impinge on critical organs, which leads to disfigurement, pain, loss of function and mobility, neurovascular compromise, and occasionally life-threatening consequences, such as mesenteric, bowel, ureteral, and/or bladder obstruction. DT, PVNS, and GCTB have few and recurrent molecular aberrations but, paradoxically, can have variable natural histories. A multidisciplinary approach is recommended for optimal management. In DT and PVNS, a course of observation may be appropriate, and any intervention should be guided by symptoms and/or disease progression. A surgical approach should take into consideration the infiltrative nature, difficulty in obtaining wide margins, high recurrence rates, acute and chronic surgical morbidities, and impact on quality of life. There are similar concerns with radiation, which especially relate to optimal field and transformation to high-grade radiation-associated sarcomas. Systemic therapies must be considered carefully in light of acute and chronic toxicities. Although standard and novel therapies are promising, many unanswered questions, such as duration of therapy and optimal end points to evaluate efficacy of drugs in clinical practice and trials, exist. Predictive biomarkers and novel clinical trial end points, such as volumetric measurement, magnetic resonance imaging T2 weighted mapping, nuclear imaging, and patient-reported outcomes, are in development and will require validation in prospective trials.

Dataset Information

Histomorphological investigation of intrahepatic connective tissue for surgical anatomy based on modern computer imaging analysis.

Background/purpose

Methods

Results

Conclusions

Publications

Histomorphological investigation of intrahepatic connective tissue for surgical anatomy based on modern computer imaging analysis.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets