Dataset Information

Profiling of RNA N ⁶ -Methyladenosine Methylation Reveals the Critical Role of m⁶A in Chicken Adipose Deposition.

ABSTRACT: One of the main objectives of broiler breeding is to prevent excessive abdominal adipose deposition. The role of RNA modification in adipose deposition is not clear. This study was aimed to map m⁶A modification landscape in chicken adipose tissue. MeRIP-seq was performed to compare the differences in m⁶A methylation pattern between fat and lean broilers. We found that start codons, stop codons, coding regions, and 3'-untranslated regions were generally enriched for m⁶A peaks. The high m⁶A methylated genes (fat birds vs. lean birds) were primarily associated with fatty acid biosynthesis and fatty acid metabolism, while the low m⁶A methylated genes were mainly involved in processes associated with development. Furthermore, we found that the mRNA levels of many genes may be regulated by m⁶A modification. This is the first comprehensive characterization of m⁶A patterns in the chicken adipose transcriptome, and provides a basis for studying the role of m⁶A modification in fat deposition.

SUBMITTER: Cheng B

PROVIDER: S-EPMC7892974 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

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Publications

Profiling of RNA N 6 -Methyladenosine Methylation Reveals the Critical Role of m6A in Chicken Adipose Deposition.

Cheng Bohan B Leng Li L Li Ziwei Z Wang Weijia W Jing Yang Y Li Yudong Y Wang Ning N Li Hui H Wang Shouzhi S

Frontiers in cell and developmental biology 20210205

One of the main objectives of broiler breeding is to prevent excessive abdominal adipose deposition. The role of RNA modification in adipose deposition is not clear. This study was aimed to map m6A modification landscape in chicken adipose tissue. MeRIP-seq was performed to compare the differences in m6A methylation pattern between fat and lean broilers. We found that start codons, stop codons, coding regions, and 3'-untranslated regions were generally enriched for m6< ...[more]

PMID: 33614638

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Project description:With the intensive development of the sheep industry and increasing global temperatures, heat stress in sheep has become an increasingly severe and important issue in recent years. The level of N6-methyladenosine (m6A) RNA methylation changes in response to stress plays important roles in stress responses. However, the role of m6A in the heat stress response of sheep remains unclear. To explore this issue, we measured heat stress protein (HSP) expression, liver function indexes, m6A on RNA, m6A-related enzyme expression, and tissue damage in sheep that had been subjected to heat stress. At the transcriptome level, our results showed significant increases in m6A on RNA and increased mRNA levels of HSPs (HSP70, HSP90, and HSP110) and m6A-related enzymes [METTL3 (methyltransferase-like 3), METTL14 (methyltransferase-like 14), WTAP (wilms tumor 1-associated protein), FTO (fat mass and obesity-associated protein), ALKBH5 (alkB homologue 5), YTHDF1-3 (YTH domain family proteins), and YTHDC1-2 (YTH domain-containing proteins)] following heat stress. At the protein level, the expression of METTL3, YTHDF1-2, and YTHDC2 showed no significant differences following heat stress. However, in contrast to its mRNA level after heat stress, the protein expression of YTHDF3 was reduced, while the expression of HSPs (HSP70, HSP90, and HSP110), METTL14, WTAP, FTO, ALKBH5, YTHDF3, and YTHDC1 increased in line with their measured mRNA levels. Histological experiments revealed that heat stress caused varying degrees of damage to sheep liver tissue. Moreover, immunohistochemical staining indicated that the m6A-related enzymes were expressed in sheep hepatocytes, and differences in expression patterns were observed between the control and heat stress groups. In summary, differences in the level of m6A and the expression of m6A-related enzymes in the liver of sheep were observed after heat stress. This indicates that m6A is involved in the regulation of heat stress in sheep. Our findings provide a new avenue for studying the responses to heat stress in sheep.

Dataset Information

Profiling of RNA N ⁶ -Methyladenosine Methylation Reveals the Critical Role of m⁶A in Chicken Adipose Deposition.

Publications

Profiling of RNA <i>N</i> <sup><i>6</i></sup> -Methyladenosine Methylation Reveals the Critical Role of m<sup>6</sup>A in Chicken Adipose Deposition.

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