Building up Pt^II -Thiosemicarbazone-Lysine-sC18 Conjugates.

ABSTRACT: Three chiral tridentate N^N^S coordinating pyridine-carbaldehyde (S)-N4-(?-methylbenzyl)thiosemicarbazones (HTSCmB) were synthesised along with lysine-modified derivatives. One of them was selected and covalently conjugated to the cell-penetrating peptide sC18 by solid-phase peptide synthesis. The HTSCmB model ligands, the HTSCLp derivatives and the peptide conjugate rapidly and quantitatively form very stable Pt^II chlorido complexes [Pt(TSC)Cl] when treated with K₂ PtCl₄ in solution. The Pt(CN) derivatives were obtained from one TSCmB model complex and the peptide conjugate complex through Cl^- ?CN^- exchange. Ligands and complexes were characterised by NMR, IR spectroscopy, HR-ESI-MS and single-crystal XRD. Intriguingly, no decrease in cell viability was observed when testing the biological activity of the lysine-tagged HdpyTSCLp, its sC18 conjugate HdpyTSCL-sC18 or the PtCl and Pt(CN) conjugate complexes in three different cell lines. Thus, given the facile and effective preparation of such Pt-TSC-peptide conjugates, these systems might pave the way for future use in late-stage labelling with Pt radionuclides and application in nuclear medicine.

SUBMITTER: Haseloer A 

PROVIDER: S-EPMC7894172 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature