Dataset Information

Effect of Traditional Chinese Medicine Poge Heart-Saving Decoction on Cardiac Function in Heart Failure Rat Model.

ABSTRACT:

Background

Poge heart-saving decoction (PHSD) has been used as a medicine treating heart failure in China for many years. The study aimed to explore the effect of PHSD on cardiac function in heart failure conditions and its underlying mechanism.

Methods

Adriamycin was used to induce the model of heart failure (HF) in rats. Sixty rats were randomly divided into six groups: blank control group, sham group, 9.33?g/kg group (low-PHSD, test group), 13.995?g/kg group (moderate-PHSD, test group), 18.66?g/kg group (high-PHSD, test group), and fosinopril group (4.67?mg/kg, comparison test group). Cardiac ultrasound was used to evaluate the cardiac function of the rats, and radioimmunoassay was used to measure aldosterone (ALD) and angiotensin II (AngII) levels in the serum.

Results

Compared with the blank control group, the left ventricular end-diastolic dimension (LVEDd) and left ventricular end-systolic dimension (LVEDs) in the sham group were increased (1.04?±?0.12 vs. 0.67?±?0.13?cm; 0.75?±?0.13 vs. 0.28?±?0.10?cm; P < 0.05), and the left ventricular ejection fraction was decreased (36.65?±?5.74 vs. 76.09?±?4.23%; P < 0.05). The ejection fraction of HF rats was increased in 9.33?g/kg group, 13.995?g/kg group, and 18.66?g/kg group compared with those of the sham group (57.13?±?1.63, 58.43?±?1.98, and 59.21?±?1.37 vs. 36.65?±?5.74%; P < 0.05). PHSD also improved cardiac function by reducing the LVEDd and LVEDs (0.88?±?0.11, 0.75?±?0.13, and 0.72?±?0.18 vs. 1.04?±?0.12?cm; 0.62?±?0.10, 0.63?±?0.17, and 0.45?±?0.11 vs. 0.75?±?0.13?cm; P < 0.05). The levels of ALD and AngII in the serum of rats in the sham group were significantly higher than those in the blank control group (371.58?±?39.25 vs. 237.12?±?17.35??g/L; 232.18?±?16.33 vs. 159.44?±?18.42?pg/L; P < 0.05). The ALD and AngII of the rats in all of the three PHSD groups and the fosinopril group were decreased (276.81?±?25.63, 277.18?±?21.35, 268.19?±?19.28, and 271.47?±?28.96 vs. 371.58?±?39.25??g/L; 169.41?±?27.53, 168.81?±?19.78, 164.23?±?21.34, and 174.27?±?22.84 vs. 232.18?±?16.33?pg/L; P < 0.05). The histopathological changes of the myocardium in the sham group showed the disorganized fiber, shaded staining, fracture, and zonation. The fracture of the myocardium was relieved in all groups except the sham group and the blank control group.

Conclusion

Therefore, PHSD could shorten LVEDd and LVEDs of rats and reverse ventricular remodeling. The mechanism might be related to the inhibition of the activation level of renin-angiotensin-aldosterone system (especially ALD and AngII) and decreasing the postload of the heart.

SUBMITTER: Liu L

PROVIDER: S-EPMC7895586 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Publications

Effect of Traditional Chinese Medicine Poge Heart-Saving Decoction on Cardiac Function in Heart Failure Rat Model.

Liu Lei L Mo Yanfei Y Wu Bingying B Yu Zongliang Z Sun Bugao B

Evidence-based complementary and alternative medicine : eCAM 20201203

<h4>Background</h4>Poge heart-saving decoction (PHSD) has been used as a medicine treating heart failure in China for many years. The study aimed to explore the effect of PHSD on cardiac function in heart failure conditions and its underlying mechanism.<h4>Methods</h4>Adriamycin was used to induce the model of heart failure (HF) in rats. Sixty rats were randomly divided into six groups: blank control group, sham group, 9.33 g/kg group (low-PHSD, test group), 13.995 g/kg group (moderate-PHSD, tes ...[more]

PMID: 33628294

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Project description:Cardiovascular diseases are a leading cause of death worldwide. After an ischemic injury, the myocardium undergoes severe necrosis and apoptosis, leading to a dramatic degradation of function. Numerous studies have reported that cardiac fibroblasts (CFs) play a critical role in heart function even after injury. However, CFs present heterogeneous characteristics according to their development stage (i.e., fetal or adult), and the molecular mechanisms by which they maintain heart function are not fully understood. The aim of this study is to explore the hypothesis that a specific population of CFs can repair the injured myocardium in heart failure following ischemic infarction, and lead to a significant recovery of cardiac function. Flow cytometry analysis of CFs defined two subpopulations according to their relative expression of vascular cell adhesion molecule 1 (VCAM1). Whole-transcriptome analysis described distinct profiles for these groups, with a correlation between VCAM1 expression and lymphangiogenesis-related genes up-regulation. Vascular formation assays showed a significant stimulation of lymphatic cells network complexity by VCFs. Injection of human VCAM1-expressing CFs (VCFs) in postinfarct heart failure rat models (ligation of the left anterior descending artery) led to a significant restoration of the left ventricle contraction. Over the course of the experiment, left ventricular ejection fraction and fractional shortening increased by 16.65% ± 5.64% and 10.43% ± 6.02%, respectively, in VCF-treated rats. Histological examinations revealed that VCFs efficiently mobilized the lymphatic endothelial cells into the infarcted area. In conclusion, human CFs present heterogeneous expression of VCAM1 and lymphangiogenesis-promoting factors. VCFs restore the mechanical properties of ventricular walls by mobilizing lymphatic endothelial cells into the infarct when injected into a rat heart failure model. These results suggest a role of this specific population of CFs in the homeostasis of the lymphatic system in cardiac regeneration, providing new information for the study and therapy of cardiac diseases.

Dataset Information

Effect of Traditional Chinese Medicine Poge Heart-Saving Decoction on Cardiac Function in Heart Failure Rat Model.

Background

Methods

Results

Conclusion

Publications

Effect of Traditional Chinese Medicine Poge Heart-Saving Decoction on Cardiac Function in Heart Failure Rat Model.

Similar Datasets

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