Analysis of lysosomal hydrolase trafficking and activity in human iPSC-derived neuronal models.
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ABSTRACT:
SUBMITTER: Cuddy LK
PROVIDER: S-EPMC7896187 | biostudies-literature | 2021 Mar
REPOSITORIES: biostudies-literature
Publications
Analysis of lysosomal hydrolase trafficking and activity in human iPSC-derived neuronal models.
Cuddy Leah K LK Mazzulli Joseph R JR
STAR protocols 20210213 1
Lysosomes are critical for maintaining protein homeostasis and cellular metabolism. Lysosomal dysfunction and disrupted protein trafficking contribute to cell death in neurodegenerative disorders, including Parkinson's disease and dementia. We describe three complementary protocols-the use of protein glycosylation, western blotting, immunofluorescence, and hydrolase activity measurement-to analyze the trafficking and activity of lysosomal proteins in patient-derived neurons differentiated from i ...[more]
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