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Protocol for assay of transposase accessible chromatin sequencing in non-model species.

ABSTRACT: The assay for transposase accessible chromatin (ATAC-seq) is a method for mapping genome-wide chromatin accessibility. Coupled with high-throughput sequencing, it enables integrative epigenomics analyses. ATAC-seq requires direct access to cell nuclei, a major challenge in non-model species such as small invertebrates, whose soft tissue is surrounded by a protective exoskeleton. Here, we present modifications of the ATAC-seq protocol for applications in small crustaceans, extending applications to non-model species. For complete information on the use and execution of this protocol, please refer to Buenrostro et al. (2013).

SUBMITTER: Kissane S

PROVIDER: S-EPMC7896190 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature

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Protocol for assay of transposase accessible chromatin sequencing in non-model species.

Kissane Stephen S Dhandapani Vignesh V Orsini Luisa L

STAR protocols 20210213 1

The assay for transposase accessible chromatin (ATAC-seq) is a method for mapping genome-wide chromatin accessibility. Coupled with high-throughput sequencing, it enables integrative epigenomics analyses. ATAC-seq requires direct access to cell nuclei, a major challenge in non-model species such as small invertebrates, whose soft tissue is surrounded by a protective exoskeleton. Here, we present modifications of the ATAC-seq protocol for applications in small crustaceans, extending applications ...[more]

PMID: 33659905

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