Ontology highlight
ABSTRACT:
SUBMITTER: Zhao J
PROVIDER: S-EPMC7897479 | biostudies-literature | 2021 Feb
REPOSITORIES: biostudies-literature
Zhao Jing J Inomata Rika R Kato Yoshio Y Miyagishi Makoto M
Nucleic acids research 20210201 3
The occurrence of accidental mutations or deletions caused by genome editing with CRISPR/Cas9 system remains a critical unsolved problem of the technology. Blocking excess or prolonged Cas9 activity in cells is considered as one means of solving this problem. Here, we report the development of an inhibitory DNA aptamer against Cas9 by means of in vitro selection (systematic evolution of ligands by exponential enrichment) and subsequent screening with an in vitro cleavage assay. The inhibitory ap ...[more]