Dataset Information

Microbial yield from infectious tissues pretreated by various methods: an invitro study.

ABSTRACT:

Background

This study aimed to evaluate the effects of different pretreatment methods on the microbial yield from infectious tissues.

Methods

Strains of Staphylococcus aureus (SA), Escherichia coli (EC) and Candida albicans (CA) were used to construct single-surface, full-surface, and internal infection models in sterile pork tissue. Manual milling (MM), mechanical homogenization (MH), sonificated (SF), dithiothreitol (DTT), and direct culture (DC) were used to pretreat these tissues, the microbial yield from different pretreatment methods were recorded and compared. Moreover, periprosthetic tissues collected intraoperatively from periprosthetic joint infection (PJI) patients were used as a verification.

Results

The study showed that the microbial yield from MH pretreatment was significantly higher than that of MM (P?ConclusionsThe effects of MH and DTT in microbial yield were significantly higher than that of DC, SF and MM, and these methods can be used to process multiple tissue samples at the same time, which might further improve the diagnostic sensitivity of infectious disease.

SUBMITTER: Cai Y

PROVIDER: S-EPMC7898421 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Microbial yield from infectious tissues pretreated by various methods: an invitro study.

Cai Yuanqing Y Fang Xinyu X Zhang Lvheng L Yang Xurong X Nie Lixiong L Huang Zida Z Li Wenbo W Zhang Chaofan C Yang Bin B Guan Zhenpeng Z Zhang Wenming W

BMC musculoskeletal disorders 20210221 1

<h4>Background</h4>This study aimed to evaluate the effects of different pretreatment methods on the microbial yield from infectious tissues.<h4>Methods</h4>Strains of Staphylococcus aureus (SA), Escherichia coli (EC) and Candida albicans (CA) were used to construct single-surface, full-surface, and internal infection models in sterile pork tissue. Manual milling (MM), mechanical homogenization (MH), sonificated (SF), dithiothreitol (DTT), and direct culture (DC) were used to pretreat these tiss ...[more]

PMID: 33612121

Similar Datasets

Project description:BackgroundDespite intensive research, malaria remains a major health concern for non-immune residents and travelers in malaria-endemic regions. Efficient adjunctive therapies against life-threatening complications such as severe malarial anaemia, encephalopathy, placental malaria or respiratory problems are still lacking. Therefore, new insights into the pathogenesis of severe malaria are imperative. Haemozoin (Hz) or malaria pigment is produced during intra-erythrocytic parasite replication, released in the circulation after schizont rupture and accumulates inside multiple organs. Many in vitro and ex vivo immunomodulating effects are described for Hz but in vivo data are limited. This study aimed to improve methods for Hz quantification in tissues and to investigate the accumulation of Hz in different organs from mice infected with Plasmodium parasites with a varying degree of virulence.MethodsAn improved method for extraction of Hz from tissues was elaborated and coupled to an optimized, quantitative, microtiter plate-based luminescence assay with a high sensitivity. In addition, a technique for measuring Hz by semi-quantitative densitometry, applicable on transmitted light images, was developed. The methods were applied to measure Hz in various organs of C57BL/6 J mice infected with Plasmodium berghei ANKA, P. berghei NK65 or Plasmodium chabaudi AS. The used statistical methods were the Mann-Whitney U test and Pearsons correlation analysis.ResultsMost Hz was detected in livers and spleens, lower levels in lungs and kidneys, whereas sub-nanomolar amounts were observed in brains and hearts from infected mice, irrespectively of the parasite strain used. Furthermore, total Hz contents correlated with peripheral parasitaemia and were significantly higher in mice with a lethal P. berghei ANKA or P. berghei NK65-infection than in mice with a self-resolving P. chabaudi AS-infection, despite similar peripheral parasitaemia levels.ConclusionsThe developed techniques were useful to quantify Hz in different organs with a high reproducibility and sensitivity. An organ-specific Hz deposition pattern was found and was independent of the parasite strain used. Highest Hz levels were identified in mice infected with lethal parasite strains suggesting that Hz accumulation in tissues is associated with malaria-related mortality.

Dataset Information

Microbial yield from infectious tissues pretreated by various methods: an invitro study.

Background

Methods

Results

Publications

Microbial yield from infectious tissues pretreated by various methods: an invitro study.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets