A Genetic Code Expansion-Derived Molecular Beacon for the Detection of Intracellular Amyloid-? Peptide Generation.

ABSTRACT: Polypeptides generated from proteolytic processing of protein precursors, or proteolytic proteoforms, play an important role in diverse biological functions and diseases. However, their often-small size and intricate post-translational biogenesis preclude the use of simple genetic tagging in their cellular studies. Herein, we develop a labeling strategy for this class of proteoforms, based on residue-specific genetic code expansion labeling with a molecular beacon design. We demonstrate the utility of such a design by creating a molecular beacon reporter to detect amyloid-? peptides, known to be involved in the pathogenesis of Alzheimer's disease, as they are produced from amyloid precursor protein (APP) along the endocytic pathway of living cells.

SUBMITTER: Sappakhaw K 

PROVIDER: S-EPMC7898502 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature