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Phospho-regulation of mitotic spindle assembly.

ABSTRACT: The assembly of the bipolar mitotic spindle requires the careful orchestration of a myriad of enzyme activities like protein posttranslational modifications. Among these, phosphorylation has arisen as the principle mode for spatially and temporally activating the proteins involved in early mitotic spindle assembly processes. Here, we review key kinases, phosphatases, and phosphorylation events that regulate critical aspects of these processes. We highlight key phosphorylation substrates that are important for ensuring the fidelity of centriole duplication, centrosome maturation, and the establishment of the bipolar spindle. We also highlight techniques used to understand kinase-substrate relationships and to study phosphorylation events. We conclude with perspectives on the field of posttranslational modifications in early mitotic spindle assembly.

SUBMITTER: Ong JY

PROVIDER: S-EPMC7898546 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

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Phospho-regulation of mitotic spindle assembly.

Ong Joseph Y JY Bradley Michelle C MC Torres Jorge Z JZ

Cytoskeleton (Hoboken, N.J.) 20201216 12

The assembly of the bipolar mitotic spindle requires the careful orchestration of a myriad of enzyme activities like protein posttranslational modifications. Among these, phosphorylation has arisen as the principle mode for spatially and temporally activating the proteins involved in early mitotic spindle assembly processes. Here, we review key kinases, phosphatases, and phosphorylation events that regulate critical aspects of these processes. We highlight key phosphorylation substrates that are ...[more]

PMID: 33280275

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