Ontology highlight
ABSTRACT:
SUBMITTER: Kampjut D
PROVIDER: S-EPMC7900225 | biostudies-literature | 2021 Mar
REPOSITORIES: biostudies-literature
Kampjut Domen D Steiner Julia J Sazanov Leonid A LA
iScience 20210205 3
Cryo-EM grid preparation is an important bottleneck in protein structure determination, especially for membrane proteins, typically requiring screening of a large number of conditions. We systematically investigated the effects of buffer components, blotting conditions and grid types on the outcome of grid preparation of five different membrane protein samples. Aggregation was the most common type of problem which was addressed by changing detergents, salt concentration or reconstitution of prot ...[more]