Ontology highlight
ABSTRACT:
SUBMITTER: Vallejo AF
PROVIDER: S-EPMC7900351 | biostudies-literature | 2021 Mar
REPOSITORIES: biostudies-literature
Vallejo Andres F AF Davies James J Grover Amit A Tsai Ching-Hsuan CH Jepras Robert R Polak Marta E ME West Jonathan J
iScience 20210205 3
Single-cell transcriptomics suffer from sensitivity limits that restrict low abundance transcript identification, affects clustering and can hamper downstream analyses. Here, we describe Constellation sequencing (Constellation-Seq), a molecular transcriptome filter that delivers two orders of magnitude sensitivity gains by maximizing read utility while reducing the data sparsity and sequencing costs. The technique reliably measures changes in gene expression and was demonstrated by resolving rar ...[more]