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Gene network transitions in embryos depend upon interactions between a pioneer transcription factor and core histones.

ABSTRACT: Gene network transitions in embryos and other fate-changing contexts involve combinations of transcription factors. A subset of fate-changing transcription factors act as pioneers; they scan and target nucleosomal DNA and initiate cooperative events that can open the local chromatin. However, a gap has remained in understanding how molecular interactions with the nucleosome contribute to the chromatin-opening phenomenon. Here we identified a short ?-helical region, conserved among FOXA pioneer factors, that interacts with core histones and contributes to chromatin opening in vitro. The same domain is involved in chromatin opening in early mouse embryos for normal development. Thus, local opening of chromatin by interactions between pioneer factors and core histones promotes genetic programming.

SUBMITTER: Iwafuchi M

PROVIDER: S-EPMC7901023 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

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Gene network transitions in embryos depend upon interactions between a pioneer transcription factor and core histones.

Iwafuchi Makiko M Cuesta Isabel I Donahue Greg G Takenaka Naomi N Osipovich Anna B AB Magnuson Mark A MA Roder Heinrich H Seeholzer Steven H SH Santisteban Pilar P Zaret Kenneth S KS

Nature genetics 20200316 4

Gene network transitions in embryos and other fate-changing contexts involve combinations of transcription factors. A subset of fate-changing transcription factors act as pioneers; they scan and target nucleosomal DNA and initiate cooperative events that can open the local chromatin. However, a gap has remained in understanding how molecular interactions with the nucleosome contribute to the chromatin-opening phenomenon. Here we identified a short α-helical region, conserved among FOXA pioneer f ...[more]

PMID: 32203463

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Gene Network Transitions in Embryos Depend Upon Interactions between a Pioneer Transcription Factor and Core Histones

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