Quantifying cell death induced by doxorubicin, hyperthermia or HIFU ablation with flow cytometry.
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ABSTRACT:
SUBMITTER: Lyon PC
PROVIDER: S-EPMC7902827 | biostudies-literature | 2021 Feb
REPOSITORIES: biostudies-literature
Publications
Quantifying cell death induced by doxorubicin, hyperthermia or HIFU ablation with flow cytometry.
Lyon Paul Christopher PC Suomi Visa V Jakeman Philip P Campo Leticia L Coussios Constantin C Carlisle Robert R
Scientific reports 20210223 1
Triggered release and targeted drug delivery of potent anti-cancer agents using hyperthermia-mediated focused-ultrasound (FUS) is gaining momentum in the clinical setting. In early phase studies, tissue biopsy samples may be harvested to assess drug delivery efficacy and demonstrate lack of instantaneous cell death due to FUS exposure. We present an optimised tissue cell recovery method and a cell viability assay, compatible with intra-cellular doxorubicin. Flow cytometry was used to determine l ...[more]
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