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Quantitative phase microscopy for non-invasive live cell population monitoring.

ABSTRACT: We present here a label-free development based on preexisting Quantitative Phase Imaging (QPI) that allows non-invasive live monitoring of both individual cells and cell populations. Growth, death, effect of toxic compounds are quantified under visible light with a standard inverted microscope. We show that considering the global biomass of a cell population is a more robust and accurate method to assess its growth parameters in comparison to compiling individually segmented cells. This is especially true for confluent conditions. This method expands the use of light microscopy in answering biological questions concerning live cell populations even at high density. In contrast to labeling or lysis of cells this method does not alter the cells and could be useful in high-throughput screening and toxicity studies.

SUBMITTER: Aknoun S

PROVIDER: S-EPMC7904828 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature

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Quantitative phase microscopy for non-invasive live cell population monitoring.

Aknoun Sherazade S Yonnet Manuel M Djabari Zied Z Graslin Fanny F Taylor Mark M Pourcher Thierry T Wattellier Benoit B Pognonec Philippe P

Scientific reports 20210224 1

We present here a label-free development based on preexisting Quantitative Phase Imaging (QPI) that allows non-invasive live monitoring of both individual cells and cell populations. Growth, death, effect of toxic compounds are quantified under visible light with a standard inverted microscope. We show that considering the global biomass of a cell population is a more robust and accurate method to assess its growth parameters in comparison to compiling individually segmented cells. This is espec ...[more]

PMID: 33627679

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