Comprehensive analysis of lncRNAs N⁶-methyladenosine modification in colorectal cancer.

ABSTRACT:

Background

Long non-coding RNAs (lncRNAs) and their N6-methyladenosine (M6A) modifications are involved in cancer occurrence and development.

Methods

lncRNA M6A modification in colorectal cancer (CRC) was comprehensively analyzed for the first time.

Results

M6A levels of lnRNAs in CRC tissues were higher than those in tumor-adjacent normal tissues. A total of 8,332 M6A peaks were detected in 6,690 lncRNAs in CRC tissues. Approximately 91% of the modified lncRNAs had unique M6A modification peaks. A total of 383 lncRNAs were differentially methylated in CRC, of which 48.24% had a length of 1-1,000 bp. Most of these were located on chromosomes 1, 2, 7, 11, 16 and 19; 42.3% were within a sense-overlapping exon. RNA sequencing identified 163 differentially expressed lncRN

SUBMITTER: Zuo L 

PROVIDER: S-EPMC7906145 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature