Unknown

Dataset Information

0

PspC domain-containing protein (PCP) determines Streptococcus mutans biofilm formation through bacterial extracellular DNA release and platelet adhesion in experimental endocarditis.


ABSTRACT: Bacterial extracellular DNA (eDNA) and activated platelets have been found to contribute to biofilm formation by Streptococcus mutans on injured heart valves to induce infective endocarditis (IE), yet the bacterial component directly responsible for biofilm formation or platelet adhesion remains unclear. Using in vivo survival assays coupled with microarray analysis, the present study identified a LiaR-regulated PspC domain-containing protein (PCP) in S. mutans that mediates bacterial biofilm formation in vivo. Reverse transcriptase- and chromatin immunoprecipitation-polymerase chain reaction assays confirmed the regulation of pcp by LiaR, while PCP is well-preserved among streptococcal pathogens. Deficiency of pcp reduced in vitro and in vivo biofilm formation and released the eDNA inside bacteria floe along with reduced bacterial platelet adhesion capacity in a fibrinogen-dependent manner. Therefore, LiaR-regulated PCP alone could determine release of bacterial eDNA and binding to platelets, thus contributing to biofilm formation in S. mutans-induced IE.

SUBMITTER: Jung CJ 

PROVIDER: S-EPMC7906467 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature

altmetric image

Publications

PspC domain-containing protein (PCP) determines Streptococcus mutans biofilm formation through bacterial extracellular DNA release and platelet adhesion in experimental endocarditis.

Jung Chiau-Jing CJ   Hsu Chih-Chieh CC   Chen Jeng-Wei JW   Cheng Hung-Wei HW   Yuan Chang-Tsu CT   Kuo Yu-Min YM   Hsu Ron-Bin RB   Chia Jean-San JS  

PLoS pathogens 20210212 2


Bacterial extracellular DNA (eDNA) and activated platelets have been found to contribute to biofilm formation by Streptococcus mutans on injured heart valves to induce infective endocarditis (IE), yet the bacterial component directly responsible for biofilm formation or platelet adhesion remains unclear. Using in vivo survival assays coupled with microarray analysis, the present study identified a LiaR-regulated PspC domain-containing protein (PCP) in S. mutans that mediates bacterial biofilm fo  ...[more]

Similar Datasets

| S-EPMC9377233 | biostudies-literature
| S-EPMC4312048 | biostudies-literature
2014-04-10 | MSV000078581 | GNPS
| S-EPMC3067281 | biostudies-literature
| S-EPMC9883903 | biostudies-literature
2014-04-10 | MSV000078578 | MassIVE
| S-EPMC3055159 | biostudies-literature
| S-EPMC6555400 | biostudies-literature
| S-EPMC9241737 | biostudies-literature
| S-EPMC4685068 | biostudies-other