Frame localisation optical projection tomography.
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ABSTRACT:
SUBMITTER: Russell CT
PROVIDER: S-EPMC7907276 | biostudies-literature | 2021 Feb
REPOSITORIES: biostudies-literature
Publications
Frame localisation optical projection tomography.
Russell Craig T CT Vallejo Ramirez Pedro P PP Rees Eric E
Scientific reports 20210225 1
We present a tomographic reconstruction algorithm (flOPT), which is applied to Optical Projection Tomography (OPT) images, that is robust to mechanical jitter and systematic angular and spatial drift. OPT relies on precise mechanical rotation and is less mechanically stable than large-scale computer tomography (CT) scanning systems, leading to reconstruction artefacts. The algorithm uses multiple (5+) tracked fiducial beads to recover the sample pose and the image rays are then back-projected at ...[more]
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