A highly sensitive octopus-like azobenzene fluorescent probe for determination of abamectin B₁ in apples.

ABSTRACT: The development of detecting residual level of abamectin B₁ in apples is of great importance to public health. Herein, we synthesized a octopus-like azobenzene fluorescent probe 1,3,5-tris (5'-[(E)-(p-phenoxyazo) diazenyl)] benzene-1,3-dicarboxylic acid) benzene (TPB) for preliminary detection of abamectin B₁ in apples. The TPB molecule has been characterized by ultraviolet-visible absorption spectrometry, ¹H-nuclear magnetic resonance, fourier-transform infrared (FT-IR), electrospray ionization mass spectroscopy (ESI-MS) and fluorescent spectra. A proper determination condition was optimized, with limit of detection and limit of quantification of 1.3 µg L^-1 and 4.4 ?g L^-1, respectively. The mechanism of this probe to identify abamectin B₁ was illustrated in terms of undergoing aromatic nucleophilic substitution, by comparing fluorescence changes, FT-IR and ESI-MS. Furthermore, a facile quantitative detection of the residual abamectin B₁ in apples was achieved. Good reproducibility was present based on relative standard deviation of 2.2%. Six carboxyl recognition sites, three azo groups and unique fluorescence signal towards abamectin B₁ of this fluorescent probe demonstrated reasonable sensitivity, specificity and selectivity. The results indicate that the octopus-like azobenzene fluorescent probe can be expected to be reliable for evaluating abamectin B₁ in agricultural foods.

SUBMITTER: Guo Z 

PROVIDER: S-EPMC7907368 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature