Dataset Information

Modulatory effect of aquaporin 5 on estrogen-induced epithelial-mesenchymal transition in prostate epithelial cells.

ABSTRACT:

Background

Estrogen is involved in the pathophysiological process of benign prostatic hyperplasia (BPH), in which epithelial-mesenchymal transition (EMT) plays an important role. Upregulation of aquaporin (AQP) 5, which is directly activated by estrogen, has been reported to promote EMT in multiple cells. This study aimed to examine the effects of AQP5 on estrogen-induced EMT in the prostate.

Methods

Normal prostate (NP) tissue samples without any histopathological changes and BPH tissue samples with pathologically confirmed hyperplasia were obtained. An EMT cell model was subsequently established by adding estradiol (E2) to RWPE-1 cells, after which AQP5 knockdown was performed. Tissue morphological and immunohistochemical features were examined using hematoxylin-eosin and immunohistochemical staining. Western blot analysis was performed to determine the expression of AQPs, estrogen receptors, and EMT-related proteins. Cell proliferation was assessed and supernatants were collected for enzyme-linked immunosorbent assay to determine transforming growth factor-?1 (TGF-?1) concentrations. Immunofluorescence staining was performed to assess protein expressions in RWPE-1 cells.

Results

BPH tissues exhibited greater EMT (TGF-?1: 1.362?±?0.196 vs. 0.107?±?0.067, P?=?0.003; vimentin: 1.581?±?0.508 vs. 0.221?±?0.047, P?ConclusionOur findings suggest that estrogen induces BPH possibly by promoting AQP5 expression. Hence, AQP5 might be a novel target for modulating EMT in prostate epithelial cells.

SUBMITTER: Fan Y

PROVIDER: S-EPMC7909481 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

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Publications

Modulatory effect of aquaporin 5 on estrogen-induced epithelial-mesenchymal transition in prostate epithelial cells.

Fan Yu Y Song Tu-Run TR Wei Qiang Q Yang Lu L Lin Tao T Feng Xiao-Bing XB Wang Xian-Ding XD Huang Zhong-Li ZL Feng Shi-Jian SJ

Chinese medical journal 20201005 4

<h4>Background</h4>Estrogen is involved in the pathophysiological process of benign prostatic hyperplasia (BPH), in which epithelial-mesenchymal transition (EMT) plays an important role. Upregulation of aquaporin (AQP) 5, which is directly activated by estrogen, has been reported to promote EMT in multiple cells. This study aimed to examine the effects of AQP5 on estrogen-induced EMT in the prostate.<h4>Methods</h4>Normal prostate (NP) tissue samples without any histopathological changes and BPH ...[more]

PMID: 33031138

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Dataset Information

Modulatory effect of aquaporin 5 on estrogen-induced epithelial-mesenchymal transition in prostate epithelial cells.

Background

Methods

Results

Publications

Modulatory effect of aquaporin 5 on estrogen-induced epithelial-mesenchymal transition in prostate epithelial cells.

Similar Datasets

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