Ontology highlight
ABSTRACT:
SUBMITTER: Liu L
PROVIDER: S-EPMC7909483 | biostudies-literature | 2019 May
REPOSITORIES: biostudies-literature
Liu Leqian L Dalal Chiraj K CK Heineike Benjamin M BM Abate Adam R AR
Lab on a chip 20190501 10
Yeast can be engineered into "living foundries" for non-natural chemical production by reprogramming them via a "design-build-test" cycle. While methods for "design" and "build" are relatively scalable and efficient, "test" remains a bottleneck, limiting the effectiveness of the procedure. Here we describe isogenic colony sequencing (ICO-seq), a massively-parallel strategy to assess the gene expression, and thus engineered pathway efficacy, of large numbers of genetically distinct yeast colonies ...[more]