Unknown

Dataset Information

0

Accelerating 3D single-molecule localization microscopy using blind sparse inpainting.


ABSTRACT:

Significance

Single-molecule localization-based super-resolution microscopy has enabled the imaging of microscopic objects beyond the diffraction limit. However, this technique is limited by the requirements of imaging an extremely large number of frames of biological samples to generate a super-resolution image, thus requiring a longer acquisition time. Additionally, the processing of such a large image sequence leads to longer data processing time. Therefore, accelerating image acquisition and processing in single-molecule localization microscopy (SMLM) has been of perennial interest.

Aim

To accelerate three-dimensional (3D) SMLM imaging by leveraging a computational approach without compromising the resolution.

Approach

We used blind sparse inpainting to reconstruct high-density 3D images from low-density ones. The low-density images are generated using much fewer frames than usually needed, thus requiring a shorter acquisition and processing time. Therefore, our technique will accelerate 3D SMLM without changing the existing standard SMLM hardware system and labeling protocol.

Results

The performance of the blind sparse inpainting was evaluated on both simulation and experimental datasets. Superior reconstruction results of 3D SMLM images using up to 10-fold fewer frames in simulation and up to 50-fold fewer frames in experimental data were achieved.

Conclusions

We demonstrate the feasibility of fast 3D SMLM imaging leveraging a computational approach to reduce the number of acquired frames. We anticipate our technique will enable future real-time live-cell 3D imaging to investigate complex nanoscopic biological structures and their functions.

SUBMITTER: Gaire SK 

PROVIDER: S-EPMC7910702 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature

altmetric image

Publications

Accelerating 3D single-molecule localization microscopy using blind sparse inpainting.

Gaire Sunil Kumar SK   Wang Yanhua Y   Zhang Hao F HF   Liang Dong D   Ying Leslie L  

Journal of biomedical optics 20210201 2


<h4>Significance</h4>Single-molecule localization-based super-resolution microscopy has enabled the imaging of microscopic objects beyond the diffraction limit. However, this technique is limited by the requirements of imaging an extremely large number of frames of biological samples to generate a super-resolution image, thus requiring a longer acquisition time. Additionally, the processing of such a large image sequence leads to longer data processing time. Therefore, accelerating image acquisi  ...[more]

Similar Datasets

| S-EPMC6179357 | biostudies-literature
| S-EPMC7612967 | biostudies-literature
| S-EPMC6378585 | biostudies-literature
| S-EPMC5428856 | biostudies-literature
| S-EPMC8080769 | biostudies-literature
| S-EPMC4242028 | biostudies-other
| S-EPMC6684258 | biostudies-literature
| S-EPMC6476321 | biostudies-literature
| S-EPMC7248114 | biostudies-literature
| S-EPMC6009849 | biostudies-literature