Concentration-Dependent Type 1 Interferon-Induced Regulation of MX1 and FABP3 in Bovine Endometrial Explants.

ABSTRACT: The inadequate maternal recognition of embryonic interferon ? (IFN?) might explain subfertility in cattle. This study aimed at modeling the inducibility of type 1 interferon receptor subunits 1/2 (IFNAR1/2), mimicking competition between IFN? and infection-associated interferon ? (IFN?), and simulating type 1 interferon pathways in vitro. Endometrial explants (n = 728 from n = 26 healthy uteri) were collected at the abattoir, challenged with IFN? and/or IFN? in different concentrations, and incubated for 24 h. Gene expression analysis confirmed the inducibility of IFNAR1/2 within this model, it being most prominent in IFNAR2 with 10 ng/mL IFN? (p = 0.001). The upregulation of interferon-induced GTP-binding protein (MX1, classical pathway) was higher in explants treated with 300 ng/mL compared to 10 ng/mL IFN? (p < 0.0001), whereas the non?classical candidate fatty acid binding protein 3 (FABP3) exhibited significant downregulation comparing 300 ng/mL to 10 ng/mL IFN?. The comparison of explants challenged with IFN? + IFN? indicated the competition of IFN? and IFN? downstream of the regulatory factors. In conclusion, using this well-defined explant model, interactions between infection-associated signals and IFN? were indicated. This model can be applied to verify these findings and to mimic and explore the embryo-maternal contact zone in more detail.

SUBMITTER: Schabmeyer ST 

PROVIDER: S-EPMC7912598 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature