?-carotene Rescues Busulfan Disrupted Spermatogenesis Through Elevation in Testicular Antioxidant Capability.

ABSTRACT: ?-carotene, precursor of vitamin A, is an excellent antioxidant with many beneficial properties. It is a lipid-soluble antioxidant and a very effective quencher of reactive oxygen species (ROS) to reduce the oxidative stress. In contrast to vitamin A, ?-carotene is not toxic even consumed in higher amount when it is delivered from natural plant products. Recently, we found that ?-carotene acts as a potential antioxidant in the oocyte to improve its quality. Even though many studies have been reported that ?-carotene has the beneficial contribution to the ovarian development and steroidogenesis, it is unknown the effects of ?-carotene on the spermatogenesis. This investigation aimed to explore the hypothesis that ?-carotene could improve spermatogenesis and the underlying mechanism. And we found that ?-carotene rescued busulfan disrupted spermatogenesis in mouse with the increase in the sperm concentration and motility. ?-carotene improved the expression of genes/proteins important for spermatogenesis, such as VASA, DAZL, SYCP3, PGK2. Moreover, ?-carotene elevated the testicular antioxidant capability by the elevation of the antioxidant glutathione and antioxidant enzymes SOD, GPX1, catalase levels. In conclusion, ?-carotene may be applied for the infertile couples by the improvement of spermatogenesis, since, worldly many couples are infertile due to the idiopathic failed gametogenesis (spermatogenesis).

SUBMITTER: Ma D 

PROVIDER: S-EPMC7917239 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature