Evaluation of Two Methods to Concentrate SARS-CoV-2 from Untreated Wastewater.
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ABSTRACT:
SUBMITTER: Dumke R
PROVIDER: S-EPMC7917696 | biostudies-literature | 2021 Feb
REPOSITORIES: biostudies-literature
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Evaluation of Two Methods to Concentrate SARS-CoV-2 from Untreated Wastewater.
Dumke Roger R de la Cruz Barron Magali M Oertel Reinhard R Helm Björn B Kallies Rene R Berendonk Thomas U TU Dalpke Alexander A
Pathogens (Basel, Switzerland) 20210212 2
Use of wastewater-based epidemiology as a tool to record and manage the course of SARS-CoV-2 infections in human populations requires information about the efficiency of methods to concentrate the virus from wastewater. In the present study, we spiked untreated wastewater with quantified SARS-CoV-2 positive clinical material and enriched the virus by polyethylene glycol precipitation and ultrafiltration with Vivaspin 10 kDa MWCO columns. SARS-CoV-2 was detected and quantified by reverse transcri ...[more]
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