Ontology highlight
ABSTRACT:
SUBMITTER: Dumke R
PROVIDER: S-EPMC7917696 | biostudies-literature | 2021 Feb
REPOSITORIES: biostudies-literature
Dumke Roger R de la Cruz Barron Magali M Oertel Reinhard R Helm Björn B Kallies Rene R Berendonk Thomas U TU Dalpke Alexander A
Pathogens (Basel, Switzerland) 20210212 2
Use of wastewater-based epidemiology as a tool to record and manage the course of SARS-CoV-2 infections in human populations requires information about the efficiency of methods to concentrate the virus from wastewater. In the present study, we spiked untreated wastewater with quantified SARS-CoV-2 positive clinical material and enriched the virus by polyethylene glycol precipitation and ultrafiltration with Vivaspin 10 kDa MWCO columns. SARS-CoV-2 was detected and quantified by reverse transcri ...[more]