Dataset Information

Marine cyanobacterium Spirulina maxima as an alternate to the animal cell culture medium supplement.

ABSTRACT: Serum is a stable medium supplement for in vitro cell culture. Live cells are used in stem cell research, drug toxicity and safety testing, disease diagnosis and prevention, and development of antibiotics, drugs, and vaccines. However, use of serum in culture involves concerns such as an ethical debate regarding the collection process, lack of standardized ingredients, and high cost. Herein, therefore, we evaluated the possibility of using edible cyanobacterium (Spirulina maxima), which is a nutrient-rich, sustainable, and ethically acceptable source, as a novel substitute for fetal bovine serum (FBS). H460 cells were cultured to the 10th generation by adding a mixture of spirulina animal cell culture solution (SACCS) and FBS to the culture medium. Cell morphology and viability, cell cycle, apoptosis, proteomes, and transcriptomes were assessed. We observed that SACCS had better growth-promoting capabilities than FBS. Cell proliferation was promoted even when FBS was replaced by 50-70% SACCS; there was no significant difference in cell shape or viability. There were only slight differences in the cell cycle, apoptosis, proteomes, and transcriptomes of the cells grown in presence of SACCS. Therefore, SACCS has the potential to be an effective, low-cost, and eco-friendly alternative to FBS in in vitro culture.

SUBMITTER: Jeong Y

PROVIDER: S-EPMC7921123 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature

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Marine cyanobacterium Spirulina maxima as an alternate to the animal cell culture medium supplement.

Jeong Younsik Y Choi Woon-Yong WY Park Areumi A Lee Yeon-Ji YJ Lee Youngdeuk Y Park Gun-Hoo GH Lee Su-Jin SJ Lee Won-Kyu WK Ryu Yong-Kyun YK Kang Do-Hyung DH

Scientific reports 20210301 1

Serum is a stable medium supplement for in vitro cell culture. Live cells are used in stem cell research, drug toxicity and safety testing, disease diagnosis and prevention, and development of antibiotics, drugs, and vaccines. However, use of serum in culture involves concerns such as an ethical debate regarding the collection process, lack of standardized ingredients, and high cost. Herein, therefore, we evaluated the possibility of using edible cyanobacterium (Spirulina maxima), which is a nut ...[more]

PMID: 33649424

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Project description:Background Microalgae represent a suitable and eco-sustainable resource for human needs thanks to their fast growth ability, together with the great diversity in species and intracellular secondary bioactive metabolites. These high-added-value compounds are of great interest for human health or animal feed. The intracellular content of these valuable compound families is tightly associated with the microalgal biological state and responds to environmental cues, e.g., light. Our study develops a Biotechnological response curve strategy exploring the bioactive metabolites synthesis in the marine cyanobacterium Spirulina subsalsa over a light energy gradient. The Relative Light energy index generated in our study integrates the red, green and blue photon flux density with their relative photon energy. The Biotechnological response curve combined biochemical analysis of the macromolecular composition (total protein, lipid, and carbohydrate content), total sterols, polyphenols and flavonoids, carotenoids, phenolic compounds, vitamins (A, B1, B2, B6, B9, B12, C, D2, D3, E, H, and K1), phycobiliproteins, together with the antioxidant activity of the biomass as well as the growth ability and photosynthesis. Results Results demonstrated that light energy significantly modulate the biochemical status of the microalga Spirulina subsalsa revealing the relevance of the light energy index to explain the light-induced biological variability. The sharp decrease of the photosynthetic rate at high light energy was accompanied with an increase of the antioxidant network response, such as carotenoids, total polyphenols, and the antioxidant capacity. Conversely, low light energy favorized the intracellular content of lipids and vitamins (B2, B6, B9, D3, K1, A, C, H, and B12) compared to high light energy. Conclusions Results of the Biotechnological response curves were discussed in their functional and physiological relevance as well as for the essence of their potential biotechnological applications. This study emphasized the light energy as a relevant tool to explain the biological responses of microalgae towards light climate variability, and, therefore, to design metabolic manipulation of microalgae. Supplementary Information The online version contains supplementary material available at 10.1186/s13068-023-02277-4.

Dataset Information

Marine cyanobacterium Spirulina maxima as an alternate to the animal cell culture medium supplement.

Publications

Marine cyanobacterium Spirulina maxima as an alternate to the animal cell culture medium supplement.

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