Ontology highlight
ABSTRACT:
SUBMITTER: Sun YH
PROVIDER: S-EPMC7921563 | biostudies-literature | 2021 Mar
REPOSITORIES: biostudies-literature
Sun Yu H YH Wang Anqi A Song Chi C Shankar Goutham G Srivastava Rajesh K RK Au Kin Fai KF Li Xin Zhiguo XZ
Nature communications 20210301 1
Sperm contributes diverse RNAs to the zygote. While sperm small RNAs have been shown to impact offspring phenotypes, our knowledge of the sperm transcriptome, especially the composition of long RNAs, has been limited by the lack of sensitive, high-throughput experimental techniques that can distinguish intact RNAs from fragmented RNAs, known to abound in sperm. Here, we integrate single-molecule long-read sequencing with short-read sequencing to detect sperm intact RNAs (spiRNAs). We identify 34 ...[more]