Electrochemical immunosensor with Cu₂O nanocube coating for detection of SARS-CoV-2 spike protein.

ABSTRACT: Severe acute respiratory syndrome SARS-CoV-2 has caused a global pandemic starting in 2020. Accordingly, testing is crucial for mitigating the economic and public health effects. In order to facilitate point-of-care diagnosis, this study aims at presenting a label-free electrochemical biosensor as a powerful nanobiodevice for SARS-CoV-2 spike protein detection. Utilizing the IgG anti-SARS-CoV-2 spike antibody onto the electrode surface as a specific platform in an ordered orientation through staphylococcal protein A (ProtA) is highly significant in fabricating the designed nanobiodevice. In this sense, the screen-printed carbon electrode modified with Cu₂O nanocubes (Cu₂O NCs), which provide a large surface area in a very small space, was applied in order to increase the ProtA loading on the electrode surface. Accordingly, the sensitivity and stability of the sensing platform significantly increased. The electrochemical evaluations proved that there is a very good linear relationship between the charge transfer resistance (R_ct) and spike protein contents via a specific binding reaction in the range 0.25 fg mL^-1 to 1 ?g mL^-1. Moreover, the assay when tested with influenza viruses 1 and 2 was performed in 20 min with a low detection limit of 0.04 fg mL^-1 for spike protein without any cross-reactivity. The designed nanobiodevice exhibited an average satisfactory recovery rate of ~ 97-103% in different artificial sample matrices, i.e., saliva, artificial nasal, and universal transport medium (UTM), illustrating its high detection performance and practicability. The nanobiodevice was also tested using real patients and healthy samples, where the results had been already obtained using the standard polymerase chain reaction (PCR) procedure, and showed satisfactory results. Graphical abstract.

SUBMITTER: Rahmati Z 

PROVIDER: S-EPMC7921825 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature